Cytiva GE Hollow Fiber Filter Tangential Flow Cartridge UFP 750 kDa

Cytiva 56-4101-78 Hollow Fiber TFF Cartridge (750 kDa, UFP-750-E-4MA)

The Cytiva 56-4101-78 (Model UFP-750-E-4MA) is a high-performance pilot-scale Tangential Flow Filtration (TFF) cartridge designed for the ultrafiltration of large biomolecules and viral particles. Featuring a 750,000 Nominal Molecular Weight Cutoff (NMWC), this cartridge utilizes Low Adsorption Polyethersulfone (PES) membrane technology to provide high flux and superior product recovery. The "Size 4" housing combined with a 1.0 mm fiber lumen makes it an ideal choice for scaling up from laboratory research to clinical production, providing the durability required for repeated use and standardized sanitation protocols.

Technical Performance and Design

• 750 kDa Ultrafiltration Membrane: This specialized cutoff is optimized for the retention of large viruses (such as Lentivirus and Adenovirus) and large protein complexes, while allowing smaller contaminating proteins and salts to pass into the permeate.

• Low Adsorption PES Media: The hydrophilic Polyethersulfone membrane is engineered to minimize fouling and non-specific binding, ensuring stable flow rates and high yields of high-value biological analytes.

• 1.0 mm "A" Lumen Diameter: The wide 1.0 mm internal fiber diameter allows the cartridge to handle higher solids concentrations and moderately viscous feed streams without the pressure spikes associated with narrower fibers.

• Pilot-Scale Efficiency: With a membrane area of approximately 420 cm², the 4MA format is designed for QuixStand and Pilot-scale systems, facilitating the processing of batches ranging from 5 to 50 Liters.

Product Specifications

• Catalog Number: 56-4101-78 (UFP-750-E-4MA)

• Cutoff (NMWC): 750,000 Daltons (750 kDa)

• Membrane Material: Low Adsorption Polyethersulfone (PES)

• Lumen ID: 1.0 mm

• Cartridge Size: Size 4 (Approx. 30 cm length)

• Connections: 1/2-inch Sanitary Flange (TC) for Process and Permeate ports.

• Sterilization: Autoclavable and compatible with Sodium Hydroxide (NaOH) sanitation.

Key Benefits & Applications

• Viral Vector Concentration: The 750 kDa membrane is widely considered the gold standard for the purification of large viral particles, providing high concentration factors with minimal shear-induced damage.

• Fractionation of Large Proteins: Ideal for separating large IgM antibodies or protein aggregates from smaller IgG molecules and albumin.

• Lysate Clarification: Provides a high-resolution separation step for removing large cellular debris and organelles while recovering soluble intracellular products.

• Linear Scale-Up: Part of the Cytiva hollow fiber family, ensuring that process parameters developed on this Size 4 module can be directly applied to large-scale production cartridges.

New; assembled with fittings; sterilized.

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