Cytiva GE Mixer Chamber 15 ml for AKTA/AVANT

Cytiva 28-9803-09 Mixer Chamber – 15 mL, for AKTA Pure & Avant Systems

The Cytiva 28-9803-09 is a precision-engineered 15 mL gradient mixer chamber designed for the AKTA Pure and AKTA Avant chromatography platforms. This high-integrity chamber is a critical component of the fluidic path, responsible for the thorough homogenization of buffers to ensure highly accurate and reproducible gradients. The 15 mL capacity is optimized for intermediate flow rates, providing the necessary volume to dampen pulsations and eliminate concentration gradients that can interfere with peak resolution. Constructed from chemically inert, high-performance materials, the chamber is designed to withstand the rigorous pressures and varied solvent conditions of modern FPLC (Fast Protein Liquid Chromatography). Whether performing ion exchange, hydrophobic interaction, or affinity chromatography, the 28-9803-09 ensures the baseline stability and gradient precision required for sophisticated protein purification protocols.

Technical Performance and Design

• High-Efficiency Mixing: Engineered to provide turbulent flow within the 15 mL volume, ensuring that buffers of different viscosities and ionic strengths are blended perfectly before entering the column.

• FPLC Compatibility: Specifically designed for the AKTA Avant and AKTA Pure 25/150 systems, featuring the standardized fittings and mounting geometry required for rapid, leak-free installation.

• Pressure Resilience: Built to operate under the high-pressure conditions of preparative chromatography, maintaining structural integrity and seal performance across the system's full operating range.

• Chemical Inertness: Manufactured from materials that are fully compatible with common aqueous buffers, high-salt solutions, and organic solvents used in protein and biomolecule purification.

Product Specifications

• Catalog Number: 28-9803-09 (28980309)

• Internal Volume: 15 mL

• Compatibility: AKTA Pure, AKTA Avant (25 and 150 models)

• Material: High-performance biocompatible polymers and glass/steel (as per OEM standards)

• Function: Static/Dynamic Inline Gradient Mixing

Key Benefits & Applications

• Reproducible Gradients: Essential for the accurate formation of linear and step gradients, which directly impacts the reproducibility of protein elution profiles and retention times.

• Preparative Chromatography: The 15 mL size is the "workhorse" volume for labs scaling up from analytical pilots to preparative protein production.

• Baseline Stability: Minimizes "noise" in UV and conductivity signals by ensuring a uniform buffer composition reaches the detectors, facilitating more accurate peak integration.

• Easy Maintenance: Designed for straightforward disassembly and cleaning, allowing for the removal of any salt precipitates or particulates that could cause backpressure issues.

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