Dionex HPLC Autosampler WPS-3000T

Dionex UltiMate 3000 WPS-3000T Thermostatted Analytical HPLC Autosampler (5820-0020)

The Dionex UltiMate 3000 WPS-3000T Thermostatted Autosampler (5820-0020) is a high-precision, temperature-controlled analytical sample injection module designed for advanced High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LC-MS) applications. Featuring an integrated peltier-based sample thermostatting engine, the WPS-3000T maintains precise sample chamber cooling and heating from 4 degrees C to 45 degrees C, preserving thermally sensitive biological samples, enzymes, and active pharmaceutical ingredients during extended sequence runs. Utilizing a low-dispersion, motorized injection valve and syringe drive mechanism, the autosampler delivers exceptional injection volume reproducibility with ultra-low sample carryover (typically under 0.005%). Capable of accommodating standard 96-well and 384-well microplates, deep-well plates, and standard 2 mL laboratory vials, this module integrates directly into Dionex UltiMate 3000 stack configurations driven by Chromeleon Chromatography Data System (CDS) software.

Technical Performance & Structural Attributes

• Integrated Peltier Sample Thermostat: Built-in cooling/heating system maintains precise sample tray temperature control from 4 degrees C to 45 degrees C to prevent thermal degradation of biological samples.
• Ultra-Low Carryover Fluidics: Optimized wash cycle fluidics and needle external/internal rinsing protocols reduce sample carryover to less than 0.005%, ensuring high mass-spectrometry quantitative accuracy.
• Flexible Sample Format Capacity: Accommodates up to 3 x 40-position 2 mL vial trays, or up to 3 x 96-well / 384-well microplates or deep-well block formats without requiring tool modifications.
• Precision Micro-Syringe Drive: High-resolution motorized syringe drive enables flexible injection modes (full loop, partial loop, and inline needle-drawn injection) across a wide range of analytical volumes.
• Dionex VIPer Zero-Dead-Volume Compatibility: Designed for seamless connection with Dionex VIPer capillary fitting systems to eliminate dead volume and maintain high chromatographic peak efficiency.

Product Specifications

• Catalog / Part Number: 5820-0020
• Model Series: UltiMate 3000 WPS-3000T
• Primary Manufacturer: Dionex (Thermo Fisher Scientific)
• Equipment Category: Thermostatted Analytical HPLC / UHPLC Autosampler
• Injection Volume Range: 0.1 µL to 100 µL (Standard); scalable up to 500 µL or 2500 µL with optional loop extensions
• Sample Temperature Control Range: 4 degrees C to 45 degrees C (minimum 22 degrees C below ambient temperature)
• Injection Precision: RSD less than 0.25% at 5 µL injection volume
• Maximum Operating Pressure: 620 bar (9,000 psi) continuous analytical operation
• Sample Capacity: 120 x 2 mL Vials or 3 x 96/384-well Microplates
• Communication Interface: System USB 2.0 / Internal System Bus
• Operating Temperature Range: 10 to 35 degrees C (15 to 80% RH Non-Condensing)
• Power Requirements: 100-240 V AC, 50/60 Hz (Auto-Sensing)

Key Benefits & Applications

• Thermolabile Sample Injections: Ideal for biopharmaceutical assays, therapeutic proteins, oligonucleotides, and clinical diagnostics requiring strict 4 degrees C sample chamber control.
• High-Throughput QA/QC & Research Sequence Runs: Enables continuous, unattended injection cycles from microplates or large vial racks for high-volume analytical testing.
• LC-MS & High-Sensitivity HPLC Front-End: Delivers clean,