Falcon Culture Flask 5 Layer with Vented Cap 875 cm2 Case of 8 Flasks

Falcon® 5-Layer Cell Culture Multi-Flasks, 875 cm² Surface Area with Vented Caps (353144) – Case of 8

The Falcon® 353144 Multi-Flask is a high-productivity, multi-layered cell culture vessel engineered for rapid biological scale-up of anchorage-dependent mammalian cell lines. Featuring an innovative 5-layer interior shelf design housed within a compact standard T-175 footprint, this vessel delivers an expansive **875 cm² of usable growth surface area**—equivalent to five standalone single-layer culture flasks. Constructed from premium optically clear virgin polystyrene, the flask distributes liquid media and cell suspensions evenly across all layers using built-in fluid partitioning mechanics. Equipped with a gas-permeable vented cap, this single-use consumable dramatically increases cellular yields per incubator shelf without requiring specialized automation hardware. Supplied as a sterile commercial case of 8 individually wrapped multi-flasks, it is ideal for scale-up operations in biomanufacturing, viral vector production, and cell therapy research workflows.

Technical Performance & Multi-Layer Fluidics

• Integrated Fluid Balancing Geometry: Engineered with unique internal manifolds that automatically segment and partition fluid volumes across the 5 structural layers. Holding the flask at specific, protocol-driven angles allows rapid, homogeneous cell seeding and media distribution without fluid trap unevenness.
• Standardized T-175 Workspace Footprint: Provides 5 times the operational surface area of a single-layer T-175 flask while utilizing identical external base dimensions. This enables seamless, high-density storage within standard CO₂ incubators and requires no modifications to existing laboratory workflow spaces.
• Direct Pipette Access & Pour Harvesting: Features a broad, straight neck entry designed to accommodate standard 10 mL serological pipettes. Technicians can smoothly aspirate media, introduce transfection reagents, or harvest detached cells directly via pipette, or utilize standard pouring protocols to speed up fluid clearance.
• Hydrophobic 0.2 µm Vent Cap Membrane: Outfitted with a premium, 32 mm threaded closure cap featuring a fused 0.2 µm hydrophobic filter matrix. This membrane permits continuous, balanced gas exchange (O₂ and CO₂) while acting as a sterile barrier against aerosolized micro-particulates and fungal or bacterial contaminants.

Product Specifications

• Catalog Number / MPN: 353144
• Primary Manufacturer: Corning® Life Sciences (Falcon® Brand Series)
• Total Growth Area Volume: 875 cm² across 5 stacked layers (175 cm² per individual shelf)
• Recommended Total Working Volume: 125 mL to 250 mL total case volume (Maximum threshold $\le 50\text{ mL}$ per layer)
• Neck Geometry Profile: Straight neck configuration with a rectangular external profile design
• Material Composition: Optical-grade, USP Class VI Virgin Polystyrene (PS) | High-clarity microscopic view profile
• Surface Treatment Chemistry: Vacuum Gas-Plasma Tissue Culture (TC) Treated for consistent cell attachment and growth uniformity
• Sterility & Purity Metrics: Gamma-irradiation sterilized (SAL $10^{-6}$ level); Certified Non-Pyrogenic and Non-Cytotoxic; Manufactured to cGMP standards
• Packaging Configuration: Case of 8 Flasks (Individually bagged inside robust, cleanroom-ready protective secondary cartons)

Key Benefits & Applications

• High-Yield Mammalian Scale-Up Operations: The premier manual scale-up choice for growing high-density adherent cell populations prior to launching large-scale automated bioreactor runs.
• Viral Vector and Vaccine Seed Seeding: Maximizes cell density per cubic foot of incubator volume, supporting elevated virus harvesting yields for downstream gene therapy and vaccine development workflows.
• Direct In-Flask Reagent Homogenization: Simplifies protocols by allowing researchers to add dissociating enzymes or transfection agents directly to the flask entry, eliminating external mixing steps and decreasing contamination risks.
• **cGMP Batch-Specific Identification:** Every multi-flask is permanently stamped with a clear, direct manufacturing lot serialization code to ensure robust compliance tracing inside strictly audited biopharmaceutical lines.

Expiration Date 2027-02-03

All product names, logos, and brands are property of their respective owners.