Fisherbrand Culture Flask 600 ml Vented Cap Case of 40 Flasks

Fisherbrand FB012939 Cell Culture Flask – 600 mL, Vented Cap, Sterile

The Fisherbrand FB012939 is a high-capacity 600 mL cell culture flask designed for the reliable expansion of adherent cell lines in research and biopharmaceutical laboratories. Manufactured from premium, optically clear virgin polystyrene, this flask features a specially treated growth surface to ensure optimal cell attachment and uniform spreading. The integrated vented cap is equipped with a 0.22 µm hydrophobic filter membrane, facilitating continuous and consistent gas exchange while maintaining a sterile environment and minimizing media evaporation. With a canted neck design for easy pipette and cell scraper access, the FB012939 is an essential tool for scaling up cultures in incubators where space efficiency and contamination control are paramount.

Technical Performance and Design

• Vacuum-Gas Plasma Surface Treatment: The internal growth surface is uniformly treated to create a hydrophilic environment, promoting superior attachment and proliferation of anchor-dependent cells.

• High-Efficiency Vented Cap: Features a breathable 0.22 µm membrane that allows for optimal CO2 and O2 exchange, ensuring stable pH levels and peak metabolic activity throughout the culture cycle.

• Canted Neck Geometry: The angled neck allows for easy access to the entire growth surface with standard pipettes or scrapers while reducing the risk of media reaching the cap during handling.

• Stackable Design: Engineered with a perimeter ridge on the top and bottom to allow for stable stacking in the incubator, maximizing vertical space without compromising airflow between flasks.

Product Specifications

• Catalog Number: FB012939

• Total Volume: 600 mL

• Growth Area: 175 cm² (T175 Equivalent)

• Material: USP Class VI Virgin Polystyrene

• Cap Style: Vented (with 0.22 µm filter)

• Sterility: Sterile (Gamma Irradiated, SAL 10⁻⁶)

• Purity: Certified Non-Pyrogenic and DNase/RNase Free

Key Benefits & Applications

• Adherent Cell Expansion: Optimized for the growth of sensitive cell lines, including HEK293, CHO, and primary human fibroblasts.

• Contamination Mitigation: The hydrophobic vent filter prevents liquid wetting and serves as a barrier against bacteria and fungi, providing a "closed" feeling to the culture environment.

• Clear Visualization: The high-clarity plastic allows for distortion-free microscopic observation of cell morphology and confluency levels.

• Scale-Up Compatibility: Provides a predictable transition from smaller T25 and T75 flasks to larger production-scale vessels like the 10-layer Cell Factory.

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