Greiner Bio-One Thincert Cell Culture Insert for 24 Well Microplate Case of 48 Inserts and 2 Plates

ThinCert® Cell Culture Inserts for 24-Well Plates, Transparent PET Membrane, 0.4 µm Pore Size (662638) – Case of 48 Inserts

The Greiner Bio-One 662638 ThinCert® Insert is a premium, research-grade cell culture consumable designed to establish multi-compartmental tissue models for transport, permeation, co-culture, and polarized monolayer assays. Precision-molded to fit standard 24-well multi-well plates, each insert incorporates a high-purity, transparent polyethylene terephthalate (PET) capillary pore membrane with a 0.4 µm track-etched pore diameter. The membrane surface undergoes a specialized physical vacuum-gas plasma modification to ensure consistent cell attachment and growth. Supplied as a commercial application case of 48 individually blister-wrapped sterile inserts, supplemented with 2 matching multi-well plates, this system is an essential tool for evaluating blood-brain barrier models, drug transport mechanics, and tissue-engineered constructs.

Technical Performance & Membrane Architecture

• Track-Etched PET Membrane Transparency: Fabricated from high-clarity, low-fluorescence polyethylene terephthalate. The 0.4 µm pore size features a defined pore density ($2 \times 10^6\text{ pores/cm}^2$), offering an optimal baseline for diffusion kinetics while physically preventing cell migration between chambers.
• Asymmetric Hanging Design Geometry: Engineered with an eccentric, hanging hanger rim configuration. This design positions the insert securely inside the receiver well while creating a dedicated pipette access clearance zone, enabling fluid sampling from the lower (basolateral) compartment without disturbing the insert.
• Advanced TC Surface Activation: The cell-contact membrane faces undergo a uniform tissue culture (TC) plasma treatment. This hydrophilic activation accelerates cell adhesion, focal adhesion point assembly, and confluent monolayer maturation across anchorage-dependent cell lines.
• Scannable In Vivo Optical Compatibility: The transparent nature of the thin PET membrane permits high-resolution phase-contrast light microscopy, immunofluorescence staging, and transepithelial electrical resistance (TEER) measurement loops directly inside the culture track.

Product Specifications

• Catalog Number / MPN: 662638
• Primary Manufacturer: Greiner Bio-One (ThinCert® Product Portfolio)
• Compatible Plate Footprint: 24-Well Microplate Matrix
• Membrane Material: High-purity, transparent Polyethylene Terephthalate (PET)
• Pore Size Diameter: 0.4 µm (Ideal for drug transport, co-culture, and secretion studies)
• Pore Density Profile: $2 \times 10^6\text{ pores/cm}^2$ nominal concentration
• Effective Growth Area: 0.33 cm² per insert unit
• Recommended Volume Capacity: Upper Insert (Apical): 0.1 mL to 0.3 mL | Lower Well (Basolateral): 0.6 mL to 1.0 mL
• Sterility Certification: Gamma-irradiated; Certified Non-Pyrogenic, Non-Cytotoxic, and free of detectable DNase, RNase, and human DNA
• Packaging Configuration: Case of 48 Inserts and 2 Plates (Individually blister-sealed sterile inserts grouped inside protective inventory packaging cartons)

Key Benefits & Applications

• Polarized Epithelial/Endothelial Layer Culturing: The standard choice for generating functional cellular barriers—such as Caco-2 intestinal layers or renal tubules—to measure directional compound transport and permeability metrics.
• Indirect Cell-to-Cell Co-Culture Paradigms: Enables researchers to culture distinct cell populations in the apical and basolateral compartments simultaneously, facilitating the study of paracrine signaling and secreted factors without direct cell contact.
• Air-Liquid Interface (ALI) Tissue Modeling: The rigid, porous PET substrate supports the lifting of epithelial cultures (such as airway or epidermal cells) to the air-liquid interface to promote differentiation and mucus production.
• **Universal Multi-Brand Well Fitment:** While supplied with 2 Greiner plates, the physical support ears are geometry-matched to establish a stable hanging mount inside most standard ANSI/SLAS compliant 24-well microplate configurations.

Expiration Date 2026/11

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