Greiner Bio-One Flask 500 ml DuoCap Baffled Bottom PC Case of 12 Flasks

Greiner Bio-One 500 mL Polycarbonate Cell Culture Shaker Flask with Baffled Bottom & White DuoCAP (679513) – Case of 12

The Greiner Bio-One 500 mL Polycarbonate Erlenmeyer Cell Culture Shaker Flask (679513) is a high-performance, sterile vessel designed for suspension cell culture, microbial fermentation, and media preparation. Engineered from optically clear, shatterproof polycarbonate (PC), this flask features an integrated baffled bottom design that increases turbulence, improves liquid-gas contact, and maximizes dissolved oxygen transfer during orbital shaking. Equipped with Greiner's patented 2-in-1 DuoCAP® system, researchers can easily switch between open venting (utilizing a breathable membrane for sterile gas exchange) and closed, leak-tight liquid storage by simple adjustment of the cap closure. Supplied pre-sterilized as a case of 12 individually wrapped flasks, this product provides clean, reproducible growth conditions for mammalian, insect, plant, and bacterial cell lines.

Technical Performance & Structural Attributes

• Integrated Baffled Bottom Geometry: Molded interior bottom baffles disrupt laminar fluid flow during orbital shaking, significantly boosting oxygenation, nutrient mixing, and culture aeration compared to flat-bottom flasks.
• Patented 2-in-1 DuoCAP® Functionality: Versatile dual-action closure operates as a breathable vented cap (with a hydrophobic membrane for continuous sterile gas exchange) or converts to a leak-tight seal for sample storage and liquid transport.
• Optically Clear Polycarbonate Resin: Provides glass-like visual transparency for clear inspection of cell density and contamination while offering impact resistance and safety against accidental drops.
• Molded Volumetric Graduations: Clear, accurate molded-in graduations allow quick liquid level monitoring and volume estimation without interfering with cell growth.
• Autoclavable & Reusable Capability: Durable polycarbonate construction supports single-use disposable workflows or can be steam autoclaved for secondary reuse.

Product Specifications

• Catalog / Part Number: 679513
• Primary Manufacturer: Greiner Bio-One
• Volumetric Working Capacity: 500 mL (Recommended working volume ~125 mL to 200 mL)
• Base Geometry: Baffled Bottom
• Cap Style: White Patented 2-in-1 DuoCAP® (Vented Membrane / Non-Vented Sealing)
• Material Composition: Pure Optically Clear Polycarbonate (PC)
• Sterility Assurance Level: Pre-Sterilized (SAL 10⁻⁶), Individually Wrapped
• Purity Profile: Certified Non-Pyrogenic, Non-Cytotoxic, RNase-free, DNase-free, and Human DNA-free
• Dimensions: ~165.3 mm Height (with cap) x 105 mm Base Outer Diameter
• Packaging Configuration: Case of 12 Flasks (1 flask per bag / 12 per case)

Key Benefits & Applications

• Suspension Cell Culture & Fermentation: Ideal for scaling up mammalian, insect, yeast, fungal, and bacterial cultures in orbital shaking incubators.
• Enhanced Oxygenation & Growth Kinetics: Baffled base increases mass transfer of oxygen, preventing hypoxia in high-density bacterial expression protocols.
• Flexible Contamination Protection: DuoCAP design prevents airborne contamination during high-speed shaking while maintaining optimal CO₂/O₂ gas interchange.
• Aseptic Workflow Security: Individually wrapped sterile packaging ensures shelf-life integrity and ready-to-use convenience in biological safety cabinets.

All product names, logos, and brands are property of their respective owners. Greiner Bio-One and DuoCAP are registered trademarks of Greiner Bio-One GmbH.