Greiner Bio-One Shaker Flask Baffled Bottom 1000 ml Polycarbonate 6 Flasks

Greiner Bio-One 679514 Erlenmeyer Shaker Flask – 1000 mL, Polycarbonate, Baffled Bottom

The Greiner Bio-One 679514 is a 1000 mL Erlenmeyer shaker flask engineered for the efficient suspension culture of mammalian cells, microbes, and plant matter. Molded from optically clear, shatter-resistant polycarbonate, this flask provides a robust alternative to fragile glass laboratory ware. A defining feature of this specific model is its baffled bottom geometry, which dramatically increases hydrodynamic agitation and oxygen transfer when placed on an orbital shaker incubator. Equipped with the proprietary DuoCAP system, this sterile, single-use flask allows researchers to seamlessly transition between vented gas exchange and a completely sealed anaerobic environment using a single closure mechanism.

Technical Performance and Design

• Baffled Bottom Geometry: The molded internal baffles disrupt laminar fluid flow during orbital shaking. This converts a simple swirl into a turbulent mixing action, driving oxygen deep into the culture media and preventing cells from settling.

• DuoCAP 2-in-1 Closure: The engineered cap features an integrated 0.2 µm PTFE membrane for sterile air exchange. By simply adjusting the screw tension, researchers can utilize it as a vented cap for aerobic cultures or tighten it fully for a secure, leak-proof seal.

• Optical Polycarbonate: The premium polycarbonate resin provides glass-like optical clarity for easy visual inspection of culture turbidity, while remaining highly shatter-resistant to ensure technician safety during high-speed agitation.

• Molded Graduations: Features prominent, molded-in volumetric graduations that will not rub off or fade, allowing researchers to quickly and accurately assess fluid levels without requiring secondary measuring vessels.

Product Specifications

• Catalog Number: 679514

• Volume Capacity: 1000 mL (1 Liter)

• Flask Material: Polycarbonate (PC)

• Bottom Profile: Baffled Base

• Closure Type: DuoCAP (0.2 µm PTFE Vented / Sealable)

• Sterility: Sterile (SAL 10^-6), Non-Pyrogenic

• Packaging: Case of 6 Flasks

Key Benefits & Applications

• Suspension Cell Culture: The rigorous mixing action created by the baffles makes it an essential vessel for high-density bacterial, yeast, or mammalian suspension cultures that require elevated dissolved oxygen levels.

• Time and Cost Efficiency: Discarding the flask after a single use entirely eliminates the labor, water, and energy costs associated with the rigorous cleaning and autoclaving cycles of traditional glass Erlenmeyer flasks.

• Cross-Contamination Prevention: Arriving sterile and strictly single-use guarantees a pristine environment for each new culture, avoiding the residual detergent or protein carryover that plagues poorly washed reusable vessels.

• Thermal Stability: Unlike standard PETG flasks, the polycarbonate construction of the 679514 allows the flask to be safely autoclaved and reused if a laboratory prefers not to dispose of it after a single run.

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