{"product_id":"invitrogen-iblot-western-detection-stacks-mini","title":"Invitrogen iBlot Western Detection Stacks Mini Pack of 10 Kits","description":"\u003ch3\u003eInvitrogen IB7010-02 iBlot Western Detection Stacks – Mini, 10 Pack\u003c\/h3\u003e\n\u003cp\u003eThe Invitrogen IB7010-02 is a set of consumable detection stacks designed exclusively for use with the iBlot Gel Transfer Device. Unlike standard transfer stacks used to move proteins from a gel to a membrane, these engineered western detection stacks utilize the iBlot device to perform rapid, electrophoretically driven immunodetection. By applying an electric field, the system actively drives charged primary and secondary antibodies directly into a nitrocellulose or PVDF membrane. This replaces standard passive diffusion incubation steps, reducing the time required for antibody-antigen binding from several hours down to approximately three minutes.\u003c\/p\u003e\n\u003ch3\u003eTechnical Performance and Design\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eElectrophoretic Antibody Delivery:\u003c\/b\u003e Uses a precisely controlled electric field to push negatively charged antibodies toward the protein-bound membrane, significantly accelerating binding kinetics compared to traditional rocking incubation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eSolid-State Gel Matrix:\u003c\/b\u003e The top and bottom stacks contain integrated copper anodes\/cathodes and a proprietary gel matrix that acts as an ion reservoir, maintaining the required pH and conductivity without liquid buffers.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eUniversal Membrane Compatibility:\u003c\/b\u003e Can be used to process both nitrocellulose and PVDF membranes, regardless of whether the initial protein transfer was performed via wet tank, semi-dry, or iBlot dry blotting methods.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eMini Format Dimensions:\u003c\/b\u003e Sized at 8 x 8 cm to accommodate standard mini-gel membrane footprints, minimizing reagent waste compared to using regular-sized stacks.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProduct Specifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eCatalog Number:\u003c\/b\u003e IB7010-02 (Often listed as IB701002)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eProduct Type:\u003c\/b\u003e Western Detection Stacks (Not Gel Transfer Stacks)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eFormat:\u003c\/b\u003e Mini Size (8 cm x 8 cm)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eSystem Compatibility:\u003c\/b\u003e iBlot Gel Transfer Device\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eDetection Mechanism:\u003c\/b\u003e Electrophoretic Immunodetection\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cb\u003ePackaging:\u003c\/b\u003e 10 Stacks per Box\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Benefits \u0026amp; Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eRapid Western Blotting:\u003c\/b\u003e Drastically cuts down total western blot processing time by eliminating the standard 1-to-2 hour passive incubation steps for primary and secondary antibodies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eBuffer-Free Workflow:\u003c\/b\u003e Eliminates the need to prepare, measure, or dispose of large volumes of running buffers, blocking solutions, or liquid antibody diluents during the primary binding phase.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eHigh-Throughput Assays:\u003c\/b\u003e Allows laboratories to process multiple membranes sequentially in a fraction of the time, increasing overall daily assay throughput.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eChromogenic and Chemiluminescent Ready:\u003c\/b\u003e Prepares the membrane for immediate downstream signal development using standard alkaline phosphatase (AP) or horseradish peroxidase (HRP) chemistries.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003cp style=\"margin: 0; font-size: 0.8em;\"\u003e\u003ci\u003eAll product names, logos, and brands are property of their respective owners.\u003c\/i\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0; font-size: 0.8em;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExpiration: December 2011\u003c\/strong\u003e\u003c\/p\u003e","brand":"Invitrogen","offers":[{"title":"Default Title","offer_id":44789854732540,"sku":"6039","price":93.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0627\/8463\/5132\/files\/invitrogen-iblot-western-detection-stacks-mini-pack-of-10-kits-filters-invitrogen-162250.jpg?v=1713468823","url":"https:\/\/lifesciencestrading.com\/products\/invitrogen-iblot-western-detection-stacks-mini","provider":"Life Sciences Trading","version":"1.0","type":"link"}