Kimble Chase Tissue Grinder 40 ml Complete Set

Kimble Chase 885300-0040 Dounce Tissue Grinder (40 ml)

The Kimble Chase 885300-0040 is a high-precision Dounce-style tissue grinder designed for the controlled disruption of cells and tissues. This 40 ml capacity unit is specifically engineered to lyse cells through gentle mechanical shearing, effectively releasing cytoplasmic contents while preserving the integrity of sensitive organelles such as nuclei, mitochondria, and chloroplasts. Manufactured from high-quality borosilicate glass, the system features a precision-machined mortar and two distinct pestles with different clearances. This dual-pestle system allows for a two-stage homogenization process, ensuring a high-yield recovery of intact intracellular components for sensitive molecular biology and biochemical assays.

Technical Performance and Design

• Precision-Fit Dual Pestle System: Includes a "Loose" pestle for initial sample reduction and a "Tight" pestle for final homogenization, providing total control over the shearing forces applied to the sample.

• Organelle Preservation: The Dounce homogenization method is preferred over high-speed blenders or ultrasonicators when the goal is to maintain the viability of nuclei or the structural integrity of enzymatic complexes.

• Type I Borosilicate Glass: Constructed from chemically inert, low-expansion glass that offers excellent resistance to thermal shock and ensures that no contaminants leach into the biological sample.

• Large 40 ml Capacity: Optimized for scale-up procedures or processing larger tissue volumes where consistent and reproducible homogenization is required across the entire batch.

Product Specifications

• Catalog Number: 885300-0040

• Volume Capacity: 40 ml

• Grinder Style: Dounce

• Material: Type I Class A Borosilicate Glass

• Included Components: 1 Mortar, 1 "Loose" Pestle, 1 "Tight" Pestle.

• Sterilization: Fully Autoclavable for sterile laboratory workflows.

Key Benefits & Applications

• Nuclear & Organelle Isolation: The primary tool for researchers performing nuclear protein extraction, chromatin immunoprecipitation (ChIP), or mitochondrial functional studies.

• Cell Fractionation: Ideal for separating cytoplasmic and nuclear fractions without significant cross-contamination from ruptured organelles.

• Soft Tissue Processing: Excellent for homogenizing liver, brain, or lymphoid tissues where cell membranes are easily disrupted by precision fluid shearing.

• Reproducible Results: The precision-ground glass surfaces ensure that the clearance between pestle and mortar remains constant, leading to standardized results between experiments.

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