Kontes Kimble Chase Tissue Grinder Pestle Set 7 ml with Pour Spout

Kimble Chase 885000-0007 Dounce Tissue Grinder Set – 7 mL, Glass, Dual Pestle

The Kimble Chase 885000-0007 is a precision-crafted 7 mL Dounce tissue grinder engineered for the gentle and controlled homogenization of animal tissues and cell suspensions. This set is fabricated from high-quality, chemically inert borosilicate glass, ensuring maximum visibility and zero sample contamination during the lysis process. The mortar tube is designed with a flared reservoir and an integrated pour spout to facilitate the aseptic, splash-free transfer of the homogenate. By utilizing a manual, vertical stroke technique, this system provides the mechanical "squeeze" necessary to rupture plasma membranes while protecting high-value organelles and protein complexes from denaturation.

Technical Performance and Design

• Dual Pestle System (Type A & B): Includes a "Loose" pestle for initial tissue reduction and a "Tight" pestle for the final lysis. This sequential approach ensures that even fibrous tissue samples can be reduced to a uniform homogenate with minimal effort.

• Integrated Pour Spout: The flared top of the mortar includes a precision-ground pour spout, allowing for the quantitative transfer of the resulting lysate to centrifuge tubes or downstream processing vessels without loss of volume.

• Premium Borosilicate Glass: Manufactured from low-extractable Type I glass. This material offers exceptional resistance to thermal shock, allowing the entire set to be autoclaved or dry-heat sterilized for RNase-free and aseptic workflows.

• Precision-Ground Clearances: The tolerances between the pestles and the mortar walls are meticulously controlled. This ensures that every 7 mL batch receives consistent mechanical force, leading to highly reproducible results across different laboratory users.

Product Specifications

• Catalog Number: 885000-0007 (Formerly Kontes)

• Product Type: Dounce Tissue Grinder

• Working Capacity: 7 mL

• Material: Class A Borosilicate Glass

• Included Components: 1 Mortar (Tube), 1 "Loose" Pestle (Type A), 1 "Tight" Pestle (Type B)

• Features: Integrated Pour Spout, Manual Operation

Key Benefits & Applications

• Subcellular Fractionation: The premier tool for isolating intact nuclei and mitochondria. The gentle action of the Dounce pestle prevents the fragmentation of these organelles, which is critical for valid metabolic and genomic studies.

• Protein & Enzyme Extraction: Because manual grinding does not generate significant heat, it is the ideal method for extracting temperature-sensitive enzymes and native-state protein complexes.

• Standardized Sample Preparation: Researchers can easily standardize their protocols by defining the number of "strokes" per sample, ensuring consistent lysis efficiency for Western Blotting, immunoprecipitation (IP), and NGS library prep.

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