{"product_id":"macherey-nagel-nucleospin-pcr-clean-up-10-preps","title":"Macherey-Nagel NucleoSpin PCR Clean-up Pack of 10 Preps","description":"\u003ch3\u003eMacherey-Nagel 740609.10 NucleoSpin Gel and PCR Clean-up Kit – 10 Preps\u003c\/h3\u003e\n\u003cp\u003eThe Macherey-Nagel 740609.10 is a NucleoSpin Gel and PCR Clean-up kit designed for the rapid isolation and purification of DNA fragments from polymerase chain reactions and agarose gels. Utilizing a silica membrane technology within a mini-spin column format, this 2-in-1 kit efficiently removes primers, nucleotides, enzymes, and salts from reaction mixtures, while also allowing for the direct dissolution and extraction of DNA from TAE or TBE agarose slices. This specific catalog number provides 10 complete preparations, making it a standard trial-size format or ideal for low-throughput operations. The proprietary Binding Buffer NTI ensures optimal pH conditions for DNA binding, enabling the recovery of fragments ranging from 50 base pairs up to 20 kilobase pairs.\u003c\/p\u003e\n\n\u003ch3\u003eTechnical Performance and Design\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eSilica Membrane Technology:\u003c\/b\u003e The engineered silica matrix strongly binds DNA in the presence of chaotropic salts, allowing enzymes, labels, and mineral oil impurities to be thoroughly washed away using the ethanolic NT3 buffer.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003e2-in-1 Flexibility:\u003c\/b\u003e Employs a single buffer system (Buffer NTI) capable of dissolving up to 200 mg of agarose gel or neutralizing up to 200 µL of PCR reaction mixtures without requiring separate protocols.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eLow Elution Volume:\u003c\/b\u003e Designed to release purified DNA with as little as 15 to 30 µL of alkaline elution buffer (Buffer NE), producing highly concentrated samples suitable for direct use in downstream enzymatic reactions.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003epH Optimized Binding:\u003c\/b\u003e The NTI binding buffer acts as a pH indicator and buffering agent, guaranteeing that the sample matrix remains at the optimal pH (5.0 to 6.0) for maximum DNA adsorption onto the silica membrane.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eProduct Specifications\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eCatalog Number:\u003c\/b\u003e 740609.10\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eFormat:\u003c\/b\u003e Mini Spin Column\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eTechnology:\u003c\/b\u003e Silica Membrane\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eSample Capacity:\u003c\/b\u003e Up to 200 µL (PCR Mixture) or up to 200 mg (Agarose Gel)\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eFragment Size Range:\u003c\/b\u003e 50 bp to 20 kbp\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eBinding Capacity:\u003c\/b\u003e 25 µg DNA\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eTypical Recovery:\u003c\/b\u003e 70% to 95%\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eElution Volume:\u003c\/b\u003e 15 µL to 30 µL\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003ePackaging:\u003c\/b\u003e 10 Preps (Spin columns and associated buffers included)\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eKey Benefits \u0026amp; Applications\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eDownstream Readiness:\u003c\/b\u003e Produces high-purity DNA (A260\/A280 ratio of 1.8 to 1.9) immediately ready for sensitive applications such as Sanger sequencing, cloning, restriction analysis, and microarray spotting.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003ePrimer Elimination:\u003c\/b\u003e Quantitatively removes unincorporated primers (\u0026lt; 50 bp) and primer-dimers from the final eluate, preventing background interference during subsequent sequencing or cloning steps.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eBroad Gel Compatibility:\u003c\/b\u003e Seamlessly dissolves both standard and low-melting-point agarose gels cast with standard electrophoresis buffers (TAE or TBE).\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp style=\"margin: 0;\"\u003e\u003cb\u003eRapid Workflow:\u003c\/b\u003e Requires only 10 minutes to process a batch of 6 samples, significantly reducing bench time compared to traditional phenol-chloroform extraction or ethanol precipitation.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003chr\u003e\n\u003cp style=\"margin: 0; font-size: 0.8em;\"\u003e\u003ci\u003eAll product names, logos, and brands are property of their respective owners.\u003c\/i\u003e\u003c\/p\u003e","brand":"Macherey-Nagel","offers":[{"title":"Default Title","offer_id":44789860565244,"sku":"6052","price":18.29,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0627\/8463\/5132\/files\/macherey-nagel-nucleospin-pcr-clean-up-pack-of-10-preps-filters-macherey-nagel-369117_Shopify1024.jpg?v=1779984508","url":"https:\/\/lifesciencestrading.com\/products\/macherey-nagel-nucleospin-pcr-clean-up-10-preps","provider":"Life Sciences Trading","version":"1.0","type":"link"}