Meso Scale Discovery Multi-Spot Plates 96 Well Pack of 2 Spot Plates

Meso Scale Discovery MULTI-SPOT Immunoassay Microplates – 96-Well, 4-Spot Small Spot Format, High-Bind Surface, Electrochemiluminescence (ECL) Multiplexing Plates (MPN: 3-2025-020002-A)

Exp Date 2020-02

The Meso Scale Discovery (MSD) 3-2025-020002-A is an instrument-grade, high-performance multi-spot microplate precision-engineered for advanced multiplexed electrochemiluminescence (ECL) immunoassays. Configured in a standard 96-well format matching ANSI/SLAS dimensions, this plate is uniquely structured with a 4-Spot pattern layout (four distinct, screen-printed carbon working electrodes clustered at the bottom of every well). Utilizing MSD's premium High-Bind surface chemistry, this plate features a specialized hydrophilic coating that drastically enhances the passive adsorption capacity of custom capture antibodies, proteins, and complex biological fractions. Optimized for simultaneous multi-analyte processing, it allows researchers to quantify up to four separate biomarkers within a single sample volume, interfacing seamlessly with MSD SECTOR Imagers and MESO QuickPlex readers.

Technical Performance and Design

• 4-Spot Multiplexed Carbon Electrodes: Outfitted with four independent carbon-ink working electrodes embedded into the floor of each well. This localized architecture enables independent electrical stimulation of separate target antibodies, allowing isolated biomarker detection without cross-reactivity or physical signal overlap.

• High-Bind Hydrophilic Surface: Chemically modified with a high-capacity, base-activated polymer layer. This hydrophilic matrix provides exceptional binding affinity for low-abundance or traditionally difficult-to-bind proteins, making it excellent for high-sensitivity homebrew multiplex assay development.

• Electrochemiluminescence (ECL) Optimization: Designed to decouple the excitation source (electrical current) from the signal detection sensor (CCD camera). This eliminates the background autoflurescence common to plastic-bottom plates, resulting in an exceptionally wide 5-log linear dynamic range.

• Sample Volume Conservation: The multi-spot multiplexing framework maximizes data density per well, allowing comprehensive multi-analyte panels to be generated from a micro-volume sample input (as low as 25 µL or less), conserving precious or rare biological fluids.

Product Specifications

• Catalog Number / MPN: 3-2025-020002-A (Also tracked under MSD Multi-Spot series family)

• Manufacturer / Brand: Meso Scale Discovery (MSD) / Meso Scale Diagnostics, LLC

• Plate Footprint Layout: Standard 96-Well Matrix Array

• Well Spot Configuration: 4-Spot Small Spot pattern array (arranged in a 2x2 internal well grid)

• Surface Binding Profile: High-Bind Hydrophilic Treatment

• Detection Technology Matrix: Electrochemiluminescence (ECL) Immunoassay

• Material Composition: Polystyrene plate frame with integrated screen-printed carbon-ink base electrodes

• Platform Hardware Compatibility: Fully compatible with the MESO QuickPlex SQ 120, MESO SECTOR S 600, MESO SECTOR S 600MM, and legacy SECTOR Imager 2400/6000 systems

Key Benefits and Applications

• Custom Multiplex Assay Development: The preferred platform for pharmaceutical R&D labs to manually spot up to four distinct target capture antibodies in-house, accelerating translational biomarker screening workflows.

• Cytokine and Chemokine Profiling: Widely used to monitor complex immune response profiles by tracking multiple cascading cytokines simultaneously out of individual cell culture supernatant fractions.

• Pharmacokinetic & Biomarker Validation: Delivers robust data reproducibility and ultra-low coefficient of variation (%CV) metrics across large clinical trial testing blocks, fulfilling validation guidelines for regulated lab workspaces.

• Robotic Liquid Handler Compatibility: Built with a highly rigid structural frame and precise physical dimensions, preventing errors or jamming during automated plate transport, barcoding, and automated plate-washing steps.

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