Meso Scale Discovery Multi-Spot Plates 96 Well Total of 10 Spot Plates

Meso Scale Discovery MULTI-SPOT Immunoassay Microplates – 96-Well, Multi-Spot Multiplex Formats, Integrated Carbon-Ink Working Electrodes, Electrochemiluminescence (ECL) Screening Plates (MPN Combo: N75XA-1 / N71XA-1)

Exp Date 2012-03 

The Meso Scale Discovery (MSD) N75XA-1 and N71XA-1 catalog codes designate a highly specialized family of instrument-grade, high-performance multi-spot microplates engineered for multi-analyte electrochemiluminescence (ECL) immunoassays. Configured in standard 96-well layouts matching ANSI/SLAS dimensions, these multi-spot plates feature specialized arrays of isolated carbon-ink working electrodes embedded at the base of every well. Designed to interface seamlessly with MSD SECTOR Imagers and MESO QuickPlex readers, these plate matrices are optimized for high-affinity biomarker capture. By isolating discrete capture zones within a single well cavity, the N75XA/N71XA series allows research laboratories to execute simultaneous multiplexed diagnostic panels—tracking distinct cytokines, proteins, or metabolites from a single raw biological sample without cross-reactivity or chemical interference.

Technical Performance and Design

• Multi-Spot Carbon Working Electrodes: Outfitted with integrated, screen-printed carbon working electrodes clustered at the baseline of each well. Applying a localized voltage triggers high-intensity light emission from bound MSD SULFO-TAG biomolecules, generating exceptionally sharp data points.

• Advanced Signal Background Decoupling: The electrochemiluminescence architecture decouples the excitation trigger (electricity) from the reading sensor (CCD camera). This layout eliminates the background autoflurescence errors common to classic plastic-bottom microplates, providing a multi-log linear dynamic range.

• Micro-Volume Sample Conservation: Maximizes data point output per well, allowing scientists to run complete multiplexed biomarker panels using minimal fluid draws (often 25 µL or less), conserving precious mouse serum, cerebrospinal fluid, or clinical cell lysates.

• Robust Surface Adsorption Parameters: Engineered with optimized surface binding characteristics that promote efficient passive coating of target antibodies, providing outstanding repeatability and ultra-low coefficient of variation (%CV) metrics across large-scale screening blocks.

Product Specifications

• Catalog Numbers / MPN: N75XA-1 / N71XA-1 (Often paired or combined as a comprehensive multi-spot diagnostic system)

• Manufacturer / Brand: Meso Scale Discovery (MSD) / Meso Scale Diagnostics, LLC

• Plate Footprint Layout: Standardized 96-Well Matrix Array

• Well Interface Profile: MULTI-SPOT Internal Clustered Target Pattern

• Detection Technology Matrix: Electrochemiluminescence (ECL) Multiplex Assay

• Material Composition: Premium Polystyrene plate chassis with embedded carbon-ink base electrodes

• Platform Hardware Compatibility: Fully compatible with the MESO QuickPlex SQ 120, MESO QuickPlex SQ 120MM, MESO SECTOR S 600, and legacy SECTOR Imager 2400/6000 systems

Key Benefits and Applications

• Multiplex Biomarker Screening: The industry-preferred tool for pharmaceutical R&D labs to capture and quantify multiple cascading cytokines or inflammation biomarkers simultaneously inside individual cell culture runs.

• Pharmacokinetic & Toxicity Profiling: Extensively deployed across translational drug development lines to chart drug efficacy, compound toxicity, and neutralizing antibody reactions in complex biological matrices.

• Unpurified Biological Matrix Compatibility: The robust carbon electrode floor handles raw, unpurified samples—such as lysed cell suspensions and tissue extracts—without premature surface fouling, minimizing laborious pre-analytical filtering steps.

• High-Velocity Automated Rigidity: Molded with highly uniform side-walls and precise skirt dimensions, preventing mechanical jams or lifting errors when manipulated by robotic gripper arms, automated fluid aspirators, and multi-plate stackers.

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