Millipore Centrifugal Filter Ultrafree-MC 0.65 um 0.5 ml PP Pack of 50 Filters

Millipore UFC30DV0S Ultrafree-MC Centrifugal Filter – 0.65 µm PVDF, 0.5 mL, Sterile

The Millipore UFC30DV0S is a high-performance, single-use centrifugal filter device engineered for the rapid clarification of low-volume biological samples. Featuring a 0.5 mL maximum capacity, this Ultrafree-MC filter incorporates a 0.65 µm hydrophilic PVDF (Durapore) membrane that delivers rapid flow rates and exceptionally low non-specific binding. This ensures maximum recovery of dilute proteins, nucleic acids, and small molecules while effectively retaining large cellular debris and precipitates. Designed to fit perfectly into standard 1.5 mL fixed-angle microcentrifuge rotors, the device utilizes centrifugal force to drive filtration, reducing processing times to mere minutes. Certified sterile and featuring an ultra-low hold-up volume, the UFC30DV0S provides researchers with a secure, highly efficient method for preparing critical samples prior to sensitive downstream analytical assays.

Technical Performance and Design

• Hydrophilic PVDF Membrane: The Durapore PVDF material provides extremely low protein and nucleic acid binding, ensuring that highly valuable or dilute biomolecules are not lost to the filter matrix during clarification.

• 0.65 µm Microfiltration: The specific 0.65-micron pore size acts as a strict physical barrier, effectively removing large particulates, cellular aggregates, and precipitates from complex biological matrices.

• Ultra-Low Hold-Up Volume: Expertly designed to retain less than 5 µL of fluid post-centrifugation, allowing researchers to recover nearly 100% of their starting material for maximum assay yield.

• Microcentrifuge Optimized: The rugged polypropylene filter housing and collection tube withstand speeds up to 12,000 x g, safely preventing structural failure or sample leakage during high-speed spins.

Product Specifications

• Catalog Number: UFC30DV0S

• Maximum Volume: 0.5 mL

• Membrane Material: Hydrophilic PVDF (Durapore)

• Pore Size: 0.65 µm

• Hold-Up Volume: < 5 µL

• Sterility & Color Code: Sterile, Purple

Key Benefits & Applications

• Pre-Analytical Clarification: The ideal sample prep tool for clearing aqueous biological solutions and cell lysates prior to downstream HPLC, LC-MS, or highly sensitive functional assays.

• Extracellular Vesicles (EVs): Frequently utilized in advanced protocols requiring the purification and isolation of exosomes or EVs from complex biofluids like seminal plasma or serum.

• Rapid Processing Time: Centrifugal force drives the sample through the membrane uniformly and quickly, vastly outperforming standard gravity-flow or syringe-driven filtration for micro-volumes.

• Cross-Contamination Prevention: The sterile, disposable format ensures that diagnostic workflows and analytical screens are never compromised by carryover from previously handled samples.

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