Millipore Centrifuge Filter 15 ml Amicon Pro with Ultra-0.5 Device 12 Filters

Millipore ACS500312 Amicon Pro Purification System – 3K MWCO, w/ Ultra-0.5 Device

The Millipore ACS500312 Amicon Pro Purification System is a revolutionary, all-in-one centrifugal device engineered to streamline the entire protein purification workflow. Unlike traditional methods that require gravity drip columns, dialysis cassettes, and multiple sample transfers, this system combines affinity purification, buffer exchange, and final concentration into one seamless spin-column process. Utilizing a 15 mL reservoir that couples directly to a 0.5 mL Amicon Ultra centrifugal filter, it allows researchers to elute their target protein directly into the concentrator. Featuring a highly retentive 3,000 Dalton Molecular Weight Cut-Off (3K MWCO) Ultracel membrane, this specific kit is the absolute gold standard for recovering, washing, and concentrating valuable low-molecular-weight proteins, small peptides, and oligonucleotides with virtually zero sample loss.

Technical Performance and Design

• All-in-One Workflow: By physically attaching the 15 mL wash/elution reservoir to the 0.5 mL concentrator, the device completely eliminates the need to pipette your precious sample from a purification column into a separate concentration tube. This drastically reduces mechanical sheer and prevents sticky proteins from being lost on plastic walls.

• Ultracel Regenerated Cellulose: The integrated Ultra-0.5 device features a premium Ultracel regenerated cellulose membrane. This ultra-low-binding material ensures maximum volumetric recovery and highly predictable concentration factors, even for dilute or hydrophobic samples.

• Simultaneous Buffer Exchange: Allows for continuous diafiltration and buffer exchange during the final concentration spin. You can rapidly clear out high-salt elution buffers or imidazole, leaving your target protein resting in its final, assay-ready storage buffer.

• High-Capacity Washing: The massive 15 mL upper reservoir allows you to heavily wash your agarose or magnetic affinity resins in a single centrifuge spin, saving hours compared to painstakingly passing wash buffers through a gravity column.

Product Specifications

• Catalog Number: ACS500312

• Product Line: Amicon Pro Purification System

• Format: 15 mL Processing Tube coupled to a 0.5 mL Concentrator

• Molecular Weight Cut-Off (MWCO): 3 kDa (3,000 Daltons)

• Membrane Material: Ultracel Regenerated Cellulose

• Typical Application: Small Peptide/Protein Affinity Purification & Concentration

Key Benefits & Applications

• Maximized Protein Yield: The absolute ideal tool for researchers working with rare, low-abundance, or highly expensive proteins where even a 5% loss during a pipette transfer could ruin an entire week's worth of cell culture work.

• Rapid Processing Time: Compresses what is normally a multi-day process (gravity affinity column followed by overnight dialysis) into a single afternoon of quick, standardized centrifuge spins.

• 3K MWCO Specificity: The tight 3 kDa pore size is explicitly designed to retain very small targets like cytokines, chemokines, and digested peptide fragments that would easily slip right through standard 10K or 30K concentrators.

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