Millipore Centrifuge Filter 4 ml 50 kDa Case of 8 Filters

Millipore Amicon Ultra-4 Centrifugal Filter Unit – 4 mL, 50 kDa MWCO, Ultracel Regenerated Cellulose Membrane, Non-Sterile (MPN: UFC805008)

The Millipore UFC805008 is a premium, high-performance Amicon Ultra-4 centrifugal ultrafiltration device engineered for the rapid concentration, desalting, purification, and buffer exchange of macromolecular biological samples up to 4 mL. This specialized system incorporates an advanced Ultracel regenerated cellulose membrane featuring a 50 kDa (50,000 Dalton) Nominal Molecular Weight Limit (NMWL) cutoff. Utilizing a patented vertical membrane alignment that minimizes solute polarization and membrane fouling, the device achieves high concentration factors of 80x to 100x with typical retentate recoveries exceeding 90 percent. Complete with a clear polystyrene filter housing and a polypropylene collection tube, this unit provides fast spin loops while safeguarding samples from spinning to absolute dryness.

Technical Performance and Design

• Anti-Fouling Vertical Membrane Layout: The internal filtration membrane is mounted vertically rather than horizontally inside the core casing. This design channels concentrated particles downward during spinning to reduce surface accumulation, preventing membrane clogging and driving ultra-fast filtration runs in as little as 5 to 15 minutes.

• Ultracel Regenerated Cellulose Matrix: Pre-packed with a heat-sealed, highly hydrophilic regenerated cellulose (RC) membrane matrix. This low-binding membrane chemistry ensures high retentate sample recoveries of macromolecules greater than 90 percent while minimizing downstream extractable interference during analytical testing.

• Built-In Physical Deadstop Zone: Engineered with a dedicated, precision-measured physical reservoir floor that maintains a minimum final concentrate volume of 50 microliters. This safety barrier stops the sample from spinning completely dry, preserving precious proteins, antibodies, or viral particles from structural denaturation.

• Direct Pipette Sample Recovery: The layout allows direct pipette tip entry into the filter core base. This architecture permits users to recover the ultra-concentrated fraction smoothly using standard pipettors without an unneeded inverted spinning loop or intermediate tube transfers.

Product Specifications

• Catalog Number / MPN: UFC805008

• Manufacturer / Brand: MilliporeSigma / Amicon Ultra-4

• Maximum Sample Processing Volume: 4.0 mL (Swinging-bucket rotor) / 3.5 mL (Fixed-angle rotor)

• Nominal Molecular Weight Limit (NMWL): 50 kDa (50,000 MWCO)

• Filter Membrane Composition: Ultracel Regenerated Cellulose (RC)

• Active Filtration Surface Area: 3 square centimeters

• Minimum Final Concentrate Volume: 50 microliters

• Color Scheme Coding: Translucent housing paired with a solid pink cap

• Sterility / Process State: Non-Sterile (Single-Use Disposable)

• Standard Pack Quantity: Pack of 8 Centrifugal Filter Assemblies

Key Benefits and Applications

• Protein Extraction and Concentration: The benchmark method for gently concentrating dilute or pre-purified proteins, monoclonal antibodies, enzymes, and nucleic acids down to microscopic volumes.

• Desalting and Buffer Exchange: Effortlessly swaps out buffers, removes excess salts, or conducts macromolecular dialysis loops by running repeated spin-wash-reconstitute cycles directly inside the vessel frame.

• HPLC Sample Purification: Cleans crude cell lysates, cellular debris, tissue culture fractions, and macro-aggregates away from target analytes to protect HPLC columns from high-pressure clogging.

• RCF Guidelines: Handles structural centrifuge stresses up to 4000 x g inside swinging-bucket rotors, and up to 7500 x g when oriented inside matching fixed-angle benchtop centrifuge heads.

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