Millipore Filter Microplate MultiScreen 96 Well 0.45 um 10 Filter Sets

Millipore MultiScreenHTS Durapore Filter Plate – 96-Well, Clear Styrene, 0.45 µm Hydrophilic PVDF

The Millipore MSHVN4510 is a premium 96-well MultiScreenHTS (High-Throughput Screening) filter plate engineered for automated liquid handling, receptor-ligand binding assays, and protein precipitation workflows. Molded from rigid, clear styrene, this plate features integrated, low-protein-binding Durapore® Hydrophilic PVDF membranes with a uniform 0.45 µm pore size. Optimized specifically for robotic systems and vacuum manifolds, its advanced two-piece design isolates individual wells with specialized "drip directors," effectively eliminating fluid wicking and cross-well contamination. A patented removable underdrain allows for direct access to the interior membranes, providing flexibility for downstream radioisotope detection, whole-organism visualization, or optical readout configurations.

Technical Performance and Design

• Durapore® Hydrophilic PVDF Membrane: The 0.45 µm membrane substrate is engineered to yield exceptionally low non-specific protein and biomolecule binding, ensuring maximum recovery of target analytes and reducing background assay noise.

• Anti-Crosstalk Well Isolation: Individual well cavities feature distinct, independent under-seals. Coupled with proprietary drip directors, the plate forces fluids straight downward, preventing lateral tracking during aggressive vacuum or centrifugation steps.

• Rigid HTS High-Skirt Skateboard: Designed with reinforced, straight sidewalls that resist bending or twisting under robotic pressure. The smooth outer flange provides a large, uniform surface area ideal for automated barcode label placement.

• Removable Underdrain System: The defensive bottom plastic underdrain can be completely snapped off, exposing the underside of the PVDF discs for direct membrane harvesting, punch-out assays, or scintillation counting.

Product Specifications

• Catalog Number / MPN: MSHVN4510

• Product Family: MultiScreenHTS® (HV Filter Code)

• Format: 96-Well Standard Layout (8 × 12 Matrix)

• Housing Material: Clear Polystyrene (Styrene)

• Membrane Chemistry: Hydrophilic Polyvinylidene Fluoride (PVDF)

• Pore Size: 0.45 µm

• Volumetric Capacity: 50 µL to 250 µL Working Range (300 µL Maximum Headspace)

• Filtration Surface Area: 0.28 cm² per well

• Sterility: Non-Sterile

• Compliance: Fully conforms to ANSI/SLAS microplate architectural dimensional standards

Key Benefits & Applications

• Receptor/Ligand Binding Assays: The industry-baseline standard for harvesting cell fragments or isolating whole-cell membranes labeled with sensitive fluorescent or radioactive tracers.

• Protein Kinase Precipitation: Highly effective for running rapid phosphoprotein or enzyme-activity precipitation assays following trichloroacetic acid (TCA) sample treatments.

• Bead- and Resin-Based Assays: Captures multiplexed microspheres, antibody-coated magnetic beads, and affinity resins while permitting rapid, high-volume wash buffer clearance.

• Manifold and Centrifuge Processing: Fully operational on standard low-pressure vacuum manifolds (optimal vacuum pressure ~305 mbar) or inside multiwell swing-out plate centrifuges up to 1,000 × g.

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