Millipore Filter Pellicon 2 Mini Cassette with 10 kD Biomax Membrane

Millipore P2B010A01 Pellicon® 2 Mini TFF Cassette (10 kD Biomax® PES, 0.1 m²)

The Millipore P2B010A01 is a high-performance Tangential Flow Filtration (TFF) cassette engineered for the concentration, desalting, and buffer exchange of proteins, enzymes, and monoclonal antibodies. This unit utilizes the 10 kD Molecular Weight Cut-Off (MWCO) Biomax® modified Polyethersulfone (mPES) membrane, renowned for its high flux rates and exceptionally low non-specific binding characteristics. Designed with the "A-Screen" (coarse-channel) feed spacer, the P2B010A01 is optimized to handle higher protein concentrations and moderately viscous solutions that would typically clog finer screens. With a 0.1 m² effective filtration area, this Pellicon® 2 Mini cassette is the primary choice for process development and pilot-scale bioproduction.

Technical Performance and Design

• Biomax® mPES Membrane: The modified polyethersulfone chemistry provides a hydrophilic surface that minimizes protein adsorption, ensuring high product recovery and easy cleaning for repeated use.

• A-Screen (Coarse Channel) Geometry: The open-channel design provides a robust flow path that reduces the risk of gel-layer formation and channel plugging, making it ideal for processing high-titer cell culture harvests and concentrated protein streams.

• Validated 10 kD Retention: Specifically engineered to provide sharp molecular weight cut-offs, making it ideal for the retention of antibodies (IgG) and other mid-sized proteins (~30 kDa to 150 kDa) while effectively removing salts and small metabolites.

• Rugged Construction: The Pellicon® 2 Mini format is built for durability and consistency, allowing for predictable scale-up to larger Pellicon® 2 industrial systems used in commercial manufacturing.

Product Specifications

• Catalog Number: P2B010A01

• Membrane Type: Biomax® (modified Polyethersulfone / mPES)

• MWCO: 10 kD

• Filtration Area: 0.1 m² (1.1 ft²)

• Feed Screen: A-Screen (Coarse / High-Concentration)

• Format: Pellicon® 2 Mini

• Operating pH Range: 1 to 14

• Sterilization: Compatible with CIP (Clean-in-Place) and sanitization using Sodium Hydroxide (NaOH).

Key Benefits & Applications

• Protein Concentration: Rapidly reduces sample volume for antibodies and recombinant proteins while maintaining protein stability and activity.

• Diafiltration & Desalting: Provides an efficient method for buffer exchange, removing residual solvents, or adjusting pH prior to final formulation.

• Monoclonal Antibody (mAb) Processing: The 10 kD pore size is the industry standard for IgG concentration and formulation steps in biopharma.

• Scalable Process Development: Identical materials and fluid dynamics to production-scale Pellicon® 2 cassettes ensure that R&D results are directly transferable to full-scale manufacturing.

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