Millipore Filter Pellicon 2 Mini Cassette with 300 kD Biomax Membrane V Screen

Millipore P2B300V01 Pellicon® 2 Mini TFF Cassette (300 kD Biomax® PES, 0.1 m²)

The Millipore P2B300V01 is a high-performance Tangential Flow Filtration (TFF) cassette engineered for the efficient concentration and purification of large molecular weight proteins, viral vectors, and vaccines. Featuring the Biomax® modified Polyethersulfone (mPES) membrane, this cassette provides high flux and exceptionally low non-specific binding. The 300 kD Molecular Weight Cut-Off (MWCO) is optimized for the retention of larger biologics while allowing smaller impurities and buffer components to pass through. Utilizing the "V-Screen" (fine-channel) feed spacer, this Pellicon® 2 Mini unit is designed to maximize mass transfer efficiency in low-to-medium viscosity solutions. With an effective filtration area of 0.1 m², it serves as the ideal scale-up tool for transitioning from laboratory research to clinical manufacturing.

Technical Performance and Design

• Biomax® mPES Membrane: The modified polyethersulfone chemistry is inherently hydrophilic, resulting in minimal protein adsorption and higher product yields compared to standard PES membranes.

• V-Screen (Fine Channel) Geometry: The optimized feed screen design promotes high turbulence at lower cross-flow rates, reducing concentration polarization and enhancing the overall permeate flux for dilute biological streams.

• Pellicon® 2 Mini Format: This compact, self-contained cassette is designed for ease of use in Pellicon® Mini holders, offering a rugged construction that withstands the pressures associated with high-performance TFF cycles.

• Reliable Scale-Up: The materials of construction and flow path dimensions are identical to larger Pellicon® 2 industrial cassettes, ensuring predictable and linear scale-up to full production volumes.

Product Specifications

• Catalog Number: P2B300V01

• Membrane Type: Biomax® (modified Polyethersulfone / mPES)

• MWCO: 300 kD

• Filtration Area: 0.1 m² (1.1 ft²)

• Feed Screen: V-Screen (Fine / Low-Viscosity)

• Format: Pellicon® 2 Mini

• Operating pH Range: 1 to 14

• Regulatory Compliance: Meets USP Class VI Biological Test for Plastics; Non-pyrogenic.

Key Benefits & Applications

• Viral Vector Processing: The 300 kD MWCO is a standard choice for the concentration and diafiltration of AAV, Lentivirus, and other viral particles used in gene therapy.

• Vaccine Manufacturing: Ideal for the purification of large antigens and virus-like particles (VLPs) from cell culture supernatants.

• Fractionation & Clarification: Provides high-efficiency separation of large macromolecular complexes from smaller plasma proteins or fermentation byproducts.

• Process Development: An essential tool for determining optimal TFF parameters (cross-flow, transmembrane pressure) before moving to larger Pellicon® 2 or Pellicon® 3 production platforms.

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