Millipore Filter Pellicon XL Cassette with Biomax Membrane 1000 kD

Millipore PXB01MC50 Pellicon® XL 50 TFF Cassette (1000 kD Biomax® PES, 50 cm²)

The Millipore PXB01MC50 is a precision Tangential Flow Filtration (TFF) device engineered for laboratory-scale concentration, clarification, and fractionation of large biological particles. Featuring the 1000 kD Biomax® modified Polyethersulfone (mPES) membrane, this cassette provides high flux and ultra-low non-specific binding for exceptionally large biomolecules. The 1000 kD Molecular Weight Cut-Off (MWCO) represents the upper range of ultrafiltration, making it an ideal choice for the retention of viral vectors, large vaccines, and nanoparticles while allowing smaller proteins and contaminants to pass into the permeate. With a 50 cm² effective filtration area, the Pellicon® XL 50 is the industry standard for researchers developing high-recovery processes with minimal sample volumes.

Technical Performance and Design

• 1000 kD Biomax® mPES Membrane: This ultra-high MWCO membrane is specifically modified to be hydrophilic, ensuring that large, sensitive biological entities do not adhere to the membrane surface, maximizing both yield and flux.

• Laboratory-Scale Efficiency: The XL 50 format is optimized for volumes as low as 100 ml, featuring low hold-up volumes to ensure maximum product recovery of high-value experimental batches.

• True Linear Scalability: Built with the same flow path materials and membrane chemistry as production-scale Pellicon® 2 and Pellicon® 3 cassettes, ensuring that process parameters developed at the 50 cm² scale can be reliably scaled up to full manufacturing levels.

• Robust Self-Contained Design: Unlike larger cassettes that require heavy stainless steel holders, the Pellicon® XL 50 is a self-contained, easy-to-use module that integrates seamlessly into benchtop TFF systems.

Product Specifications

• Catalog Number: PXB01MC50

• Membrane Type: Biomax® (modified Polyethersulfone / mPES)

• MWCO: 1000 kD (1 MDa)

• Filtration Area: 50 cm²

• Format: Pellicon® XL 50

• Max Operating Pressure: 5.5 bar (80 psi) at 25 °C

• Regulatory Compliance: Meets USP Class VI Biological Test for Plastics; Non-pyrogenic.

Key Benefits & Applications

• Viral Vector Concentration: Ideal for the concentration and diafiltration of large viral particles (such as Lentivirus or Adenovirus) where high recovery and rapid processing are essential.

• Clarification of Cell Lysates: Frequently used for the "primary clarification" step to remove large cellular debris and organelles while allowing the target protein to pass through the 1000 kD pores.

• Nanoparticle & Liposome Processing: Provides a gentle, low-shear environment for the washing and concentration of drug-delivery nanoparticles, maintaining particle size and polydispersity.

• Vaccine Development: Optimized for the purification of large antigens and virus-like particles (VLPs) from complex fermentation or cell culture streams.

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