Millipore Pellicon 2 Mini Cassette with Biomax Membrane 300 kDa

Millipore P2B300C01 Pellicon 2 Mini TFF Cassette (Biomax 300 kDa, 0.1 m2)

The Millipore P2B300C01 is a high-performance Tangential Flow Filtration (TFF) cassette engineered for the concentration, desalting, and fractionation of large biological molecules. This cassette features the Biomax polyethersulfone (PES) membrane, which is renowned for its high flux, low protein binding, and broad chemical compatibility. With a 300 kDa MWCO, it is optimized for processing large-bore feedstreams where high retention of viruses, large multi-meric proteins, or nanoparticles is required. The Pellicon 2 Mini hardware design ensures linear scalability to larger Pellicon systems, making it an essential tool for process development and small-to-medium scale biopharmaceutical production.

Technical Performance and Design

• Biomax PES Membrane Technology: The modified polyethersulfone (PES) chemistry provides a hydrophilic surface that minimizes fouling and non-specific binding, resulting in exceptionally high product recovery and consistent flux rates.

• 300 kDa MWCO Selection: Ideally tuned for the concentration of viral particles (such as AAV or Lentivirus), large proteins (>1000 kDa), and the removal of smaller host cell proteins and salts.

• C-Screen Configuration: Utilizes a "C" (coarse) feed screen geometry, which is designed to handle moderately viscous fluids and higher solids concentrations without excessive pressure drop, ensuring stable transmembrane pressure (TMP).

• Precision Engineered Flow Path: The internal manifold design ensures uniform fluid distribution across the 0.1 m2 membrane surface, maximizing efficiency and preventing localized "dead zones" where samples could aggregate.

Product Specifications

• Catalog Number: P2B300C01

• Membrane Type: Biomax (PES)

• MWCO: 300 kDa

• Filtration Area: 0.1 m2 (1.1 ft2)

• Screen Type: C-Screen (Coarse)

• Hardware Compatibility: Fits Pellicon 2 Mini holders and equivalent TFF manifolds.

• Storage Condition: Typically supplied in 0.1N NaOH or a similar preservative to maintain membrane hydration.

Key Benefits & Applications

• Viral Vector Processing: A staple in Gene Therapy workflows for the concentration and diafiltration of viral vectors, providing the necessary pore size to retain the virus while clearing smaller contaminants.

• Vaccine Production: Used in the purification of larger viral antigens and recombinant protein vaccines where high-throughput and high-yield recovery are critical.

• Scalable Process Development: The Pellicon 2 Mini format is designed to mimic the fluid dynamics of larger 0.5 m2 and 2.5 m2 cassettes, allowing for a seamless transition from the bench to cGMP manufacturing.

• Robust Cleaning & Sanitization: The Biomax membrane is highly resistant to sodium hydroxide (NaOH), permitting multiple cleaning cycles for improved cost-efficiency in non-disposable workflows.

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