Millipore Pellicon XL Cassette with Biomax Membrane 300 kDa

Millipore PXB300C50 Pellicon XL TFF Cassette (Biomax 300 kDa, 50 cm2)

The Millipore PXB300C50 is a self-contained Tangential Flow Filtration (TFF) device engineered for micro-scale concentration, desalting, and buffer exchange of high-value biological solutions. This cassette utilizes the Biomax polyethersulfone (PES) membrane, renowned for its exceptionally low protein binding and high flux. With a 300 kDa Molecular Weight Cut-Off (MWCO), it is optimized for the retention of large biomolecules, including viruses, viral vectors (AAV, Lentivirus), and large protein complexes. The Pellicon XL format is designed for "plug-and-play" simplicity; its encapsulated design eliminates the need for a separate cassette holder, allowing for efficient processing of samples in volumes ranging from 100 mL to 2 liters.

Technical Performance and Design

• Biomax PES Membrane: The modified polyethersulfone chemistry provides a hydrophilic surface that reduces fouling and ensures high product recovery (>95%), even with sensitive or low-concentration samples.

• 300 kDa Selective Barrier: Engineered for the concentration of viral particles and large multi-meric proteins while allowing host cell proteins, salts, and smaller contaminants to be cleared into the permeate.

• Encapsulated Hardware: The device is fully encapsulated in a robust plastic housing, providing a secure, leak-proof closed system that can be easily integrated into benchtop pump systems using standard tubing and fittings.

• True TFF Scalability: Despite its small size, the Pellicon XL maintains the same fluid dynamics and membrane chemistry as larger Pellicon systems (Mini and Maxi), ensuring that bench-scale results can be accurately scaled up to pilot and production volumes.

Product Specifications

• Catalog Number: PXB300C50

• Membrane Type: Biomax (PES)

• MWCO: 300 kDa

• Filtration Area: 50 cm2 (0.005 m2)

• Hold-up Volume: < 3.2 mL (Total hold-up volume), allowing for high concentration factors.

• Max Operating Pressure: 5.5 bar (80 psi) @ 25°C; 2.1 bar (30 psi) @ 50°C.

Key Benefits & Applications

• Gene Therapy & Virology: An ideal tool for researchers concentrating AAV or Lentiviral vectors where small batch sizes and high yield recovery are critical.

• Antibody & Protein Purification: Used for the rapid concentration and diafiltration of monoclonal antibodies (mAbs) and large recombinant proteins.

• Desalting & Buffer Exchange: Provides a faster and more efficient alternative to stirred-cell filtration or traditional dialysis for volumes under 2 liters.

• Reduced Setup Time: Because no holder is required, the Pellicon XL significantly reduces the time spent on equipment setup and cleaning, facilitating faster project turnaround.

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