Millipore Vacuum Filter Tube Steriflip 50 ml Pack of 25 Filters

MilliporeSigma® Steriflip®-GP Sterile Centrifuge Tube-Top Vacuum Filter Unit, 50 mL, 0.22 µm PES (SCGP00525) – Pack of 25

The MilliporeSigma® Steriflip®-GP (SCGP00525) is a specialized, closed-system disposable vacuum filtration device engineered for the rapid clarification and sterilization of small-volume biological fluids and tissue culture media. Utilizing a unique tube-top connection mechanics, the device threads directly onto a standard 50 mL conical centrifuge tube containing the raw sample, eliminating messy liquid transfer steps and reducing the risk of benchtop spillage or accidental contamination. Outfitted with a high-performance 0.22 µm Millipore Express® polyethersulfone (PES) membrane, this device provides rapid flow rates and exceptionally low protein binding, collecting the sterile filtrate straight into an attached companion 50 mL storage tube.

Technical Performance & Design

• Closed-System Flip Filtration: Designed to attach directly to the sample-containing 50 mL tube. Once threaded tight, the entire assembly is inverted ("flipped") and connected to a standard laboratory vacuum line via an integrated dual-ported adapter block, minimizing ambient air exposure.
• High-Flow Millipore Express® PES Membrane: Features a premium 0.22 micron polyethersulfone membrane matrix that maximizes hydraulic fluid flow. It exhibits exceptionally low non-specific protein adsorption, ensuring high recovery of downstream target analytes, growth factors, and sensitive proteins.
• Elimination of Fluid Transfer Spillage: Because the filtration path occurs strictly inside the interconnected tube housing, it completely removes the need to pour unsterile fluids into traditional open-funnel receiver cups, saving processing time and avoiding biohazard aerosols.
• Multi-Tube Ergonomic Compatibility: Standard threading metrics interface cleanly with major brands of 50 mL conical centrifuge tubes, while the package includes specialized sterile collection tubes and caps to secure long-term cold storage.

Product Specifications

• Model Number / SKU: SCGP00525 (Steriflip®-GP Series)
• Primary Manufacturer: MilliporeSigma® / EMD Millipore Corp.
• Maximum Processing Capacity: 50 mL Volumetric Working Limit
• Membrane Pore Size Metric: 0.22 µm (Optimized for sterile baseline filtration)
• Membrane Material Chemistry: Millipore Express® Polyethersulfone (PES)
• Filter Surface Area: $7.3\text{ cm}^2$ Effective Filtration Area (EFA)
• Sterility Specification: Gamma Radiation Sterilized Baseline
• Purity Verification: Certified non-pyrogenic and biocompatible for delicate cell line maintenance
• Packaging Configuration: Pack of 25 Filter Units (Individually blister-packed, includes clear filtrate storage tubes and leak-proof caps)

Key Benefits & Applications

• Tissue Culture Media Sterilization: Highly recommended for clarifying and filtering small batches of mammalian cell culture media, serum-supplemented mixtures, and complex growth matrices inside a laminar flow hood.
• High-Purity Analytical Buffer Clarification: Excellent for prepping mobile phases, salt-heavy buffers, and protein reagents prior to sensitive chromatography runs, preventing downstream column particulate blockages.
• Cellular Debris and Precipitate Separation: Safely isolates single cells or small biological components from dense cellular aggregates, sticky debris, or macro-precipitates following enzymatic tissue digestion.
• Radioactive and Biohazardous Sample Containment: The completely sealed flow loop provides an essential layer of laboratory safety when handling toxic fractions or radioisotopes, preventing hazardous spills or airborne leaks.

MilliporeSigma®, Steriflip®, and Millipore Express® are registered commercial trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. Part number SCGP00525 specifies the sterile 0.22 µm polyethersulfone formulation. For large-scale multi-liter batches or processes requiring coarse particle screening, alternative vacuum-driven bottle-top filter assemblies or nylon net screen filters should be specified instead.