Miltenyi Biotec Pre-Separation Filter 70 um Sealed 50 Filters

Miltenyi Biotec® Pre-Separation Filters 70 µm, Purple (130-095-823) – Case of 50

The Miltenyi Biotec® 130-095-823 is a highly specialized cell preparation consumable engineered for the efficient removal of cell aggregates, clumps, and large debris from single-cell suspensions. Featuring a 70 µm mesh pore width, these filters are structurally optimized to prep samples prior to downstream fluidics workloads, safeguarding delicate downstream hardware and ensuring a uniform cell stream. Color-coded purple for immediate mesh size recognition, these filters are supplied pre-sterilized and ready to use in a **case of 50 individually packed filters** to maintain strict aseptic conditions throughout sensitive processing workflows.

Technical Performance & Design

• High-Yield 70 µm Pore Matrix: Outfitted with an exact 70 µm nylon mesh screen that isolates single cells while capturing clumped matrices, cellular fragments, and macro-particulates that can skew quantitative data.
• Targeted Column & Tube Interfacing: Custom molded to sit securely on top of MACS® magnetic separation columns (including MS, LS, LD, CS, Large Cell, and Whole Blood configurations) as well as industry-standard 5 mL and 13–15 mL laboratory tubes.
• Fluid Dynamic Venting Footprint: The internal geometry provides an optimized fluid flow path, allowing steady, gravity-assisted filtration of tissue dissociates without causing vapor-lock pressure or vacuum resistance.
• Hydrophobic Membrane Conditioning: The filtration membrane features a slight inherent hydrophobicity. Wetting the mesh with 500 µL of standard matching buffer prior to loading allows immediate, smooth capillary flow of cell suspensions.

Product Specifications

• Model Number / SKU: 130-095-823 (MACS® Sample Prep Series)
• Primary Manufacturer: Miltenyi Biotec® B.V. & Co. KG
• Mesh Pore Dimension: 70 µm (70 micron metric baseline)
• Color-Coded Classification: Purple (Differentiates from 20 µm Green and 30 µm Yellow variants)
• Recommended Sample Working Volume: 500 µL to 3,500 µL ($\approx 0.5\text{ to }3.5\text{ mL}$)
• Sterility Status: Pre-Sterilized, Aseptically Sealed
• Regulatory Use Baseline: For Research Use Only (RUO); Not for therapeutic or direct human in vivo infusion
• Packaging Configuration: Case of 50 Filters (Individually wrapped, sterile ready-to-use units)

Key Benefits & Applications

• MACS® Magnetic Cell Separation Prep: Vital for filtering single-cell suspensions derived from primary tissues before loading onto magnetic columns, avoiding matrix clogging and maintaining high purity yields.
• Flow Cytometry & Cell Sorting Protection: Highly recommended for clearing tissue homogenates or blood cell matrices before flow running, completely protecting fragile cytometer nozzles from blockages.
• Tissue Dissociation Post-Processing: Provides an efficient filtration step directly following a gentleMACS™ Dissociator run to screen out remaining connective tissue strands from digested lung, tumor, or brain samples.
• High Macromolecule Protection: Helps keep post-nuclear supernatants (PNS) or genomic extractions uniform, providing clean liquid phases for downstream biochemical isolation.

Miltenyi Biotec®, MACS®, and gentleMACS™ are registered trademarks of Miltenyi Biotec B.V. & Co. KG. Part number 130-095-823 specifies the 70 µm purple variant. For larger volume processing utilizing 50 mL conical tubes or requiring stackable mesh grading steps, alternative MACS® SmartStrainers should be integrated instead.