NanoString nCounter Master Kit Prep Pack Preparation for 192 Reactions

NanoString nCounter Master Kit Prep Pack – 192 Assay Capacity, Multi-Chamber Sample Processing Consumables, Digital Analyzer Multiplexing Support (MPN: NAA-AKIT-192)

Expiration: 2021.

The NanoString NAA-AKIT-192 is an instrument-grade, high-throughput molecular diagnostic consumable suite engineered for the automated fluidic preparation, purification, and immobilization of target nucleic acids on the nCounter® Analysis System (including the nCounter Pro and MAX/FLEX platforms). Scaled for a full 192-sample assay throughput, this comprehensive Prep Pack serves as the operational substrate for automated fluidic workstations. It contains all the structural microfluidic consumables required to process single-molecule digital quantification workflows—including specialized racked pipet tips, foil piercers, 12-sample strip tubes, matching strip tube caps, cartridge well seals, tube sheaths, and proprietary nCounter hybridization buffer. This system enables the direct, amplification-free multiplex counting of up to 800 unique RNA, DNA, or protein transcripts simultaneously while ensuring crisp baseline imaging validation.

Technical Performance and Design

• Zero-Amplification Direct Digital Counting: Tailored to isolate target-specific ternary complexes without requiring traditional RT-PCR enzymatic amplification or cDNA synthesis steps. This eliminates enzymatic bias and preserves accurate gene expression counts from low-input biological samples.

• Comprehensive 192-Assay Consumable Core: Bundles all essential high-throughput liquid-handling mechanics—including precision-molded racked pipette tips, secure foil piercers, strip tubes, sheaths, and cartridge seals—into a unified part configuration to prevent cross-component mismatch errors.

• Optimized Hybridization Buffering: Accompanied by pre-measured, quality-controlled nCounter Hybridization Buffer. This chemical matrix promotes highly specific, background-free hybridization between target strands and fluorescent color-coded barcode probe pairs.

• Robotic Liquid-Handler Integration: Every component features strict ANSI/SLAS physical layout specifications, ensuring smooth grid seating, flawless puncture metrics for foil piercers, and error-free pick-and-place tracking by automated liquid-handling arms.

Product Specifications

• Catalog Number / MPN: NAA-AKIT-192 (Associated with the nCounter Pro Master Kit Series)

• Manufacturer / Brand: NanoString Technologies, Inc. (A Bruker Spatial Biology Brand)

• Total Processing Capacity: 192 Standard Assays / Target Reactions

• Kit Component Array: Racked pipette tips, foil piercers, 12-sample strip tubes, strip tube closures, tip sheaths, cartridge well sealing strips, and liquid hybridization buffer

• Target Instrument Compatibility: Calibrated for exclusive automated use inside the nCounter Prep Station, nCounter Pro Analysis System, and Digital Analyzer platforms

• Supported Biological Inputs: Highly compatible with purified total RNA, crude cell or blood lysates, fresh/frozen fractions, and severely degraded Formalin-Fixed Paraffin-Embedded (FFPE) tissue biopsies

Key Benefits and Applications

• High-Plex Translational Genotyping: The primary preparation framework utilized by oncology core facilities and genomics labs to run highly multiplexed immune response, inflammation, and breast cancer panels.

• FFPE Tissue Biopsy Screening: Enables precise, reproducible molecular profiling from small-volume, highly degraded clinical tissue arcs, utilizing 100-base-pair contiguous probe targets that bypass formal chemical extraction limitations.

• Unattended Multi-Plate Throughput: Rigid structural framing allows the automated Prep Station to process multiple rows of sample strip tubes and cartridges unattended, maximizing workflow efficiency in busy reference testing facilities.

• Signal Cross-Talk Defended: The clear, high-density plastics are certified free of background auto-fluorescence, ensuring the downstream digital imager CCD camera records sharp, high-contrast barcode images over a wide 5-log dynamic range.

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