Phenomenex Strata Microplate Impact Protein Precipitation 2 ml 96 Well 2 Plates

Phenomenex Strata Impact Protein Precipitation Microplate – 2 mL, 96-Well Square

The Phenomenex CE0-7565 is a high-performance Strata Impact Protein Precipitation (PPT) microplate engineered to provide a rapid, highly efficient method for removing proteins from plasma and tissue samples. The defining feature of this 2 mL, 96-well square filter plate is Phenomenex's proprietary Solvent Shielding Technology. Unlike conventional filter plates that immediately leak when organic solvents are added, the oleophobic membrane in the Impact plate successfully holds aggressive precipitation solvents (like acetonitrile or methanol) above the filter for up to 25 minutes. This allows researchers to perform direct, in-well protein precipitation—combining and mixing the sample and solvent directly inside the plate—completely eliminating the need for offline vortexing and messy sample transfer steps.

Technical Performance and Design

• Solvent Shielding Technology: The specially treated oleophobic membrane completely prevents organic solvents and biological samples from prematurely dripping through the filter. Fluid only passes through when a vacuum, centrifuge, or positive pressure manifold is explicitly applied.

• Direct In-Well Precipitation: By allowing the 3:1 or 4:1 organic-to-aqueous crash to occur directly inside the filter well, it cuts sample handling time in half and significantly reduces the risk of cross-contamination and manual pipetting errors.

• Maximized Analyte Recovery: The filter membranes are explicitly passivated to prevent non-specific binding, ensuring that critical target analytes (especially sensitive or hydrophobic drugs) are not trapped in the frit during the final elution step.

• High-Capacity 2 mL Square Wells: The deep 2 mL square well geometry safely accommodates up to 1.6 mL of total combined liquid (e.g., 400 µL of plasma and 1.2 mL of solvent) without the risk of well-to-well cross-talk or splashing during vigorous in-tip mixing.

Product Specifications

• Catalog Number: CE0-7565

• Format: 96-Well Square Filter Plate

• Separation Mode: Protein Precipitation (PPT)

• Maximum Well Volume: 2 mL

• Maximum Processing Volume: 1.6 mL combined liquid per well

• Leak Resistant Time: Up to 25 minutes (without vacuum/pressure)

• Packaging: 2 Plates per Box

Key Benefits & Applications

• Clinical & Pharmacokinetic Assays: The industry standard for rapidly crashing out dense proteins from human plasma, serum, or whole blood prior to sensitive LC-MS/MS or GC-MS screening.

• High-Throughput Automation: The standard SBS footprint is perfectly optimized for automated liquid handling robots, allowing for rapid dispense/aspirate mixing cycles directly in the well without manual intervention.

• Prolongs Analytical Column Life: Yields a remarkably clean, protein-depleted filtrate that actively prevents the clogging, high backpressure, and ion suppression that typically ruin expensive analytical chromatography columns.

• Simplified Workflow: Replaces tedious offline centrifuge-and-transfer protocols with a seamless "add, mix, and push" workflow, scaling up laboratory throughput.

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