Phenomenex Strata-X Microelution Method Dev 96 Well Sealed Plate

Phenomenex Strata-X Microelution Method Dev Microplate – Peptide Screening, 2 mg/well

The Phenomenex KS0-9528 is a highly specialized Solid Phase Extraction (SPE) microplate designed to accelerate method development for the extraction of complex peptides and small molecules. Configured in a high-throughput microelution format, this single 96-well plate is uniquely pre-loaded with two different polymeric sorbents side-by-side: 6 rows (48 wells) of Strata-X-CW (Weak Cation Exchange) and 6 rows (48 wells) of Strata-X-A (Strong Anion Exchange). Each well contains a minimal 2 mg sorbent bed. This dual-chemistry configuration allows analytical chemists to simultaneously screen different extraction mechanisms to determine the highest recovery rate for their target peptides. Furthermore, the microelution design enables elution in incredibly small volumes (as low as 25 µL), completely eliminating the time-consuming and loss-prone dry-down and reconstitution steps prior to LC-MS/MS injection.

Technical Performance and Design

• Dual-Sorbent Screening: Features 48 wells of Strata-X-CW and 48 wells of Strata-X-A, allowing researchers to test multiple retention mechanisms simultaneously under identical matrix conditions to quickly pinpoint the optimal extraction protocol.

• Microelution Efficiency (2 mg): The ultra-low 2 mg sorbent mass is explicitly optimized for micro-volume biological samples. It allows for highly concentrated elution volumes (25–50 µL), entirely bypassing the nitrogen dry-down step that frequently causes adsorptive losses of "sticky" peptides to the walls of collection plates.

• Advanced Polymeric Backbone: Both Strata-X sorbents feature a rugged, surface-modified polymeric base that resists deconditioning. Accidental drying under a vacuum manifold will not ruin the sorbent bed or compromise sensitive extraction recoveries.

• Direct-to-Vial Workflow: The concentrated extracts can be eluted directly into an analytical collection plate or autosampler vial insert, streamlining the transition from sample preparation directly to the mass spectrometer.

Product Specifications

• Catalog Number: KS0-9528

• Format: 96-Well Microelution Plate

• Sorbent Type 1: Strata-X-CW (Polymeric Weak Cation Exchange) – 6 Rows

• Sorbent Type 2: Strata-X-A (Polymeric Strong Anion Exchange) – 6 Rows

• Sorbent Mass: 2 mg per well

• Target Application: Peptide Screening and Method Development

Key Benefits & Applications

• Accelerated Method Development: Drastically reduces the time and biological sample volume required to lock in a validated SPE method for novel pharmaceutical peptides or clinical biomarkers.

• Peptide Recovery Optimization: Because peptides frequently possess varying isoelectric points and zwitterionic properties, testing both cation and anion exchange simultaneously helps identify the best wash and elution conditions for complex matrices.

• LC-MS/MS Ready: Directly addresses the severe ion-suppression challenges of modern mass spectrometry by aggressively stripping away phospholipids and salts while keeping final extract volumes highly concentrated.

• High-Throughput Automation: The standard SBS footprint is perfectly optimized for automated liquid handling robots, positive pressure manifolds, and rapid batch processing without manual intervention.

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