Repligen Conical Bottom Bottle 250 ml with 4 Port Cap 2 Bottles

Repligen ACBT-250-F2N Conical Bottom Reservoir – 250 mL, 4-Port, 4 Dip Tubes

The Repligen ACBT-250-F2N is a precision-engineered 250 mL process vessel designed specifically for small-volume Tangential Flow Filtration (TFF) and cross-flow applications. Unlike standard flat-bottom bottles, this reservoir features a steeply sloped conical bottom that funnels the liquid down to a central point. The specialized cap features four distinct ports, each equipped with its own internal dip tube that reaches precisely into the conical base. This design allows for complex fluid handling—such as simultaneous feed draw, retentate return, buffer addition, and sampling—without introducing air into the lines or causing excess shear. It is an essential component for researchers working with KrosFlo or similar benchtop TFF systems to concentrate high-value viral vectors, exosomes, and recombinant proteins.

Technical Performance and Design

• Conical Bottom Geometry: The sloped base directs all fluid to the lowest central point, allowing the system to run at exceptionally low hold-up volumes and ensuring maximum recovery of precious, low-yield samples.

• 4-Port Multi-Tasking: The four independent ports allow for the simultaneous management of the feed stream, retentate return loop, diafiltration buffer addition, and sterile venting, all within a single closed vessel.

• Extended Dip Tubes: Each of the four ports includes a dip tube cut to reach the absolute bottom of the cone. This enables submerged fluid return, which drastically reduces foaming and protein denaturation associated with splashing.

• Biocompatible Construction: Manufactured from high-purity, medical-grade plastics that provide excellent chemical compatibility and low non-specific protein binding.

Product Specifications

• Catalog Number: ACBT-250-F2N

• Capacity: 250 mL

• Vessel Profile: Conical Bottom

• Cap Configuration: 4-Port Cap

• Internal Plumbing: 4 Dip Tubes

• System Compatibility: Benchtop TFF Systems (e.g., KrosFlo KR2i)

Key Benefits & Applications

• Extreme Sample Concentration: The conical design is the gold standard for final-stage concentration steps, allowing researchers to push their concentration factors to the absolute limit without running the pump dry.

• Foam Prevention: Because the retentate is returned below the surface of the liquid via the dip tubes, it prevents the generation of foam, protecting sensitive monoclonal antibodies and enzymes from shear stress.

• Continuous Diafiltration: The multi-port setup allows for automated, constant-volume buffer exchange, drastically speeding up sample preparation and formulation steps.

• R&D Scale Bioprocessing: Perfectly sized for benchtop pilot studies, process optimization, and scaling calculations before moving to larger pilot-plant filtration skids.

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