Repligen MicroKros Hollow Fiber Filter 500 kDa

Repligen C04-E500-05-N MicroKros Hollow Fiber Filter – 500 kD, 40 cm², mPES

The Repligen C04-E500-05-N MicroKros filter module is an advanced, small-scale tangential flow filtration (TFF) device engineered for the rapid concentration and diafiltration of biological solutions. Utilizing a highly hydrophilic Modified Polyethersulfone (mPES) membrane, this module delivers exceptional flux rates and remarkably low non-specific protein binding, ensuring maximum recovery of target molecules. With a 500 kD molecular weight cut-off and a 40 cm² effective surface area, this specific filter is perfectly tailored for processing laboratory-scale volumes (10 mL to 250 mL) of large complex biomolecules, viruses, and nanoparticles. The MicroKros design ensures a gentle, low-shear processing environment that preserves the structural integrity and biological activity of sensitive samples.

Technical Performance and Design

• Advanced mPES Membrane: The modified polyethersulfone chemistry is inherently hydrophilic, reducing membrane fouling and providing higher, more consistent flux rates compared to standard PES or cellulose materials.

• 500 kD MWCO: The large pore size is meticulously controlled to retain massive macromolecular structures (like viral particles and vesicles) while allowing smaller host cell proteins and buffers to pass freely into the permeate.

• Gentle Processing: The hollow fiber geometry creates a low-shear fluid path, which is critical for maintaining the viability and titer of fragile biological assemblies that could be damaged by centrifugal forces or stirred cells.

• Direct Scalability: Built with a 41.5 cm effective length, it mirrors the path length of larger pilot and production-scale modules, allowing for accurate process modeling and seamless linear scale-up.

Product Specifications

• Catalog Number: C04-E500-05-N

• Filter Line: MicroKros

• Molecular Weight Cut-Off (MWCO): 500 kD

• Membrane Material: Modified Polyethersulfone (mPES)

• Effective Surface Area: 40 cm²

• Fiber Inner Diameter (ID): 0.5 mm

• Effective Length: 41.5 cm

Key Benefits & Applications

• Gene Therapy & Virology: The 500 kD size is an industry standard for the concentration and purification of lentiviral vectors, adenoviruses, and adeno-associated viruses (AAV).

• Exosome Isolation: Provides an efficient, scalable alternative to ultracentrifugation for the harvesting and washing of extracellular vesicles from cell culture supernatants.

• Nanoparticle Processing: Ideal for the diafiltration and buffer exchange of lipid nanoparticles (LNPs) and polymeric drug delivery vehicles.

• R&D Efficiency