Repligen MidiKros Hollow Fiber Filter mPES 300 kDa

Repligen MidiKros D06-E300-05-S Hollow Fiber Filter – Sterile mPES, 300 kDa, 370 cm2

The Repligen D06-E300-05-S is a precision-engineered MidiKros hollow fiber module designed for laboratory-scale Tangential Flow Filtration (TFF). Utilizing modified Polyethersulfone (mPES) membrane technology, this filter offers high flux rates and exceptional product recovery with minimal non-specific binding. This "S" designation indicates that the module is supplied Gamma Irradiated (Sterile), providing a ready-to-use, closed-system solution for aseptic bioprocessing. With a 300 kDa MWCO and an extended 370 cm2 surface area, it is optimized for the concentration and diafiltration of mid-volume biological samples, including viral vectors, enzymes, and larger recombinant proteins.

Technical Performance and Design

• Modified PES Membrane: Proprietary mPES chemistry provides a hydrophilic, low-fouling surface that maximizes water permeability and ensures high product recovery (>95% typical) compared to traditional PES.

• Increased Surface Area: The 65 cm effective length provides 370 cm2 of filtration area, allowing for higher total throughput and shorter processing times for batches up to 5 liters.

• 300 kDa Selective Barrier: Ideally suited for the concentration of viral particles and the fractionation of large proteins from smaller host cell contaminants and salts.

• Gamma Irradiated (Sterile): Supplied sterile and ready for immediate integration into aseptic flow paths, reducing setup time and the risk of batch contamination.

Product Specifications

• Catalog Number: D06-E300-05-S

• Module Family: MidiKros

• MWCO: 300 kDa

• Surface Area: 370 cm2

• Effective Length: 65 cm

• Fiber ID: 0.5 mm

• Connections: Female Luer Lock (FLL) Inlet, Retentate, and Permeate ports.

• Sterility: Gamma Irradiated (Sterile)

Key Benefits & Applications

• AAV & Lentivirus Concentration: Frequently utilized in gene therapy research for the sterile concentration and buffer exchange of viral vectors.

• Protein Purification: Excellent for the desalting and volume reduction of recombinant proteins and monoclonal antibodies (mAbs).

• Aseptic Process Development: Provides a sterile environment for laboratory-scale process optimization, ensuring a smooth transition to pilot and production scales.

• Gentle Processing: The hollow fiber geometry ensures low-shear filtration, preserving the biological activity of shear-sensitive molecules.

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