Repligen MiniKros Hollow Fiber Filter 750 kDa 235 cm2

Repligen MidiKros D04-E750-05-N Hollow Fiber Filter – mPES, 750 kDa, 235 cm2

The Repligen D04-E750-05-N is a high-performance MidiKros Sampler hollow fiber module designed for laboratory-scale Tangential Flow Filtration (TFF). Utilizing proprietary modified Polyethersulfone (mPES) membrane technology, this filter offers high flux rates and exceptional product recovery with minimal non-specific binding. This "N" designation indicates that the module is supplied dry and non-sterile, making it an ideal, cost-effective option for applications where user-led sanitization is preferred or for non-aseptic laboratory research. With a 750 kDa MWCO and a 235 cm2 surface area, it is optimized for the concentration and diafiltration of small-volume biological samples, including viral vectors, large multi-meric proteins, and nanoparticles.

Technical Performance and Design

• Modified PES Membrane Technology: The mPES chemistry provides a hydrophilic, low-fouling surface that ensures high water permeability and high product yield, making it superior to standard PES for sensitive biologicals.

• 750 kDa Selective Barrier: Specifically tuned for large-molecule Ultrafiltration, providing high retention of viral particles (AAV/Lenti) while effectively clearing smaller host cell proteins and buffer components.

• Optimized Laboratory Geometry: The 0.5 mm fiber ID (lumen) and 41.5 cm effective length allow for precise control of shear forces, ensuring gentle processing for shear-sensitive viruses and large proteins.

• Versatile Luer Lock Connections: Features standardized Female Luer Lock (FLL) ports, facilitating rapid and secure integration with common laboratory TFF tubing sets and benchtop pump systems.

Product Specifications

• Catalog Number: D04-E750-05-N

• Module Family: MidiKros Sampler

• MWCO: 750 kDa

• Surface Area: 235 cm2

• Effective Length: 41.5 cm

• Fiber ID: 0.5 mm

• Connections: Female Luer Lock (FLL) Inlet/Outlet/Permeate

• Sterility: Non-Sterile (Supplied Dry)

Key Benefits & Applications

• Process Development: An economical choice for membrane compatibility studies and optimizing TFF parameters before moving to sterile production-scale modules.

• Viral Vector Concentration: Extensively used in R&D for the concentration of AAV and Lentiviral vectors where sterile packaging is not required for the specific experimental phase.

• Desalting & Buffer Exchange: Ideal for rapid protein cleaning and volume reduction of recombinant protein batches ranging from 100 mL to 3 Liters.

• Full Scalability: Part of the Spectrum filtration line, ensuring that performance data generated with this MidiKros module is directly applicable to larger MiniKros and KrosFlo production systems.

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