Repligen MiniKros Hollow Fiber Filter 750 kDa 41.5cm2

Repligen MidiKros D04-E750-05-S Hollow Fiber Filter – Sterile mPES, 750 kDa, 235 cm2

The Repligen D04-E750-05-S is a precision-engineered MidiKros hollow fiber module designed for laboratory-scale Tangential Flow Filtration (TFF). Utilizing modified Polyethersulfone (mPES) membrane technology, this filter offers high flux rates and exceptional product recovery with minimal non-specific binding. This "S" designation indicates that the module is supplied Gamma Irradiated (Sterile), providing a ready-to-use, closed-system solution for aseptic bioprocessing. With a 750 kDa MWCO and a 235 cm2 surface area, it is optimized for the concentration and diafiltration of small-volume biological samples, including viral vectors, large multi-meric proteins, and nanoparticles.

Technical Performance and Design

• Aseptic Ready (-S): Gamma irradiated for immediate use in sterile workflows, eliminating the time and resource requirements for pre-use sanitization of the filter module.

• Advanced mPES Membrane: The modified Polyethersulfone chemistry provides a hydrophilic, low-fouling surface that ensures high water permeability and excellent recovery of sensitive biomolecules.

• 750 kDa Selective Barrier: Specifically tuned for large-molecule Ultrafiltration, providing high retention of viral particles while effectively clearing smaller host cell proteins and buffer components.

• Laboratory Scale Geometry: The 0.5 mm fiber ID and 41.5 cm effective length provide optimal shear conditions for volumes ranging from 100 mL to 3 Liters, ensuring gentle processing of shear-sensitive samples.

Product Specifications

• Catalog Number: D04-E750-05-S

• Module Family: MidiKros

• MWCO: 750 kDa

• Surface Area: 235 cm2

• Effective Length: 41.5 cm

• Fiber ID: 0.5 mm

• Connections: Female Luer Lock (FLL) ports for easy integration with standard lab-scale TFF tubing sets.

• Sterility: Gamma Irradiated (Sterile)

Key Benefits & Applications

• Viral Vector Concentration: A premier choice for R&D-scale concentration of AAV and Lentiviral vectors where maintaining sterile integrity is paramount.

• Protein Purification: Ideal for the desalting and concentration of large recombinant proteins and vaccines prior to analytical testing or downstream steps.

• Nanoparticle Processing: Provides the controlled shear and sterile environment necessary for the clarification and buffer exchange of lipid nanoparticles and exosomes.

• Scalable Development: The MidiKros platform allows for a seamless transition to MiniKros and KrosFlo production-scale modules by maintaining consistent membrane chemistry and path lengths.

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