Repligen Spectrum Hollow Fiber Filter 0.2 um 5200 cm2

Repligen Spectrum Hollow Fiber Filter – 0.2 µm, 5200 cm², MPN: M22M-600-01N

The Repligen Spectrum M22M-600-01N is a premium Tangential Flow Filtration (TFF) hollow fiber cartridge precision-engineered for pilot-to-production scale microfiltration, downstream bioprocessing, and target molecule clarification. This single-use module features a high-flux, modified Polyethersulfone (mPES) membrane matrix with a strict 0.2 µm pore classification. Offering an expansive 5,200 cm² active surface area and an inner lumen structure optimized for gentle fluid dynamics, the cartridge is designed to cross-flow fluid layers tangentially across the membrane surface. This configuration effectively limits gel-layer accumulation and membrane fouling, making it an exceptional alternative to legacy flat-sheet cassettes or traditional dead-end filters during high-biomass concentrations.

Technical Performance and Design

• Advanced mPES Membrane Chemistry: Formulated with a hydrophilic modified polyethersulfone composite that minimizes non-specific protein adsorption and membrane fouling, delivering up to 3x higher water permeability and superior selectivity compared to standard polysulfone alternatives.

• High-Flux Hollow Fiber Architecture: Fluid moves rapidly through the parallel hollow fiber tubes. Targeted cells and large macromolecular aggregates remain safely bound in the retentate loop while purified liquids or small target particles pass through the 0.2 µm pores into the permeate stream.

• Low Shear Processing: The smooth, open-lumen fluid channels generate a highly protective, low-shear mechanical profile, safeguarding delicate, shear-sensitive target matrices (such as encapsulated mRNA, LNPs, viral vectors, or mammalian cell lines) from mechanical lysis or structural denaturation.

• Scalable Plug-and-Play Assembly: Engineered to provide direct scale-up predictability from small-scale development models, ensuring seamless integration with existing pilot automated TFF skids and continuous manufacturing lines.

Product Specifications

• Catalog Number / MPN: M22M-600-01N

• Manufacturer: Repligen Corporation (Spectrum® Product Line)

• Filtration Surface Area: 5200 cm² (0.52 m²)

• Pore Size Classification: 0.2 µm (Microfiltration Grade)

• Membrane Material: Modified Polyethersulfone (mPES)

• Filtration Type: Tangential Flow Filtration (TFF) / Cross-Flow

• Sterilization Baseline: Non-Irradiated (Can be flushed/sanitized per manufacturer SOPs)

Key Benefits & Applications

• Cell Culture Clarification: The industry baseline standard for the rapid harvesting and separation of whole mammalian cells, yeast suspensions, or dense bacterial lysates from nutritious growth media.

• Sterile Liquid Filtration: The precise 0.2 µm cutoff safely removes bacteria, mold spores, and large ambient microparticulates from bulk biological buffer mixtures, media fractions, and vaccine lots.

• Biologics Concentration & Diafiltration: Extensively utilized in down-stream processing lines for concentrating large biomolecules, viral vectors (such as AAV, Lentivirus), and plasmid DNA (pDNA) templates.

• High Volumetric Product Recovery: Yields consistent product recoveries exceeding 95%, optimizing overall processing speeds and significantly compressing manufacturing batch cycles compared to traditional dead-end depth filtration setups.

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