Slide-A-Lyzer Dialysis Cassette 12 ml 2K MWCO Pack of 8 Cassettes

Thermo Scientific 66212 Slide-A-Lyzer™ Dialysis Cassette (12 ml, 2K MWCO)

The Thermo Scientific 66212 is a precision dialysis vessel engineered for the secure and efficient buffer exchange, desalting, and purification of low-molecular-weight proteins and peptides. Featuring a 2,000 Dalton (2K) Molecular Weight Cut-Off (MWCO) regenerated cellulose membrane, this cassette ensures the retention of small biomolecules while allowing for the rapid diffusion of salts and contaminants. The unique cassette architecture provides a high surface-area-to-volume ratio, significantly outperforming traditional dialysis tubing in both speed and ease of use. This leak-proof, high-recovery system is designed for sample volumes between 3 ml and 12 ml, making it a critical tool for researchers working with delicate, low-mass biological samples.

Technical Performance and Design

• 2K MWCO Regenerated Cellulose: Optimized for the retention of peptides and small proteins, the high-purity membrane offers excellent chemical compatibility and ultra-low non-specific binding for maximum sample recovery.

• Rapid Dialysis Kinetics: The dual-membrane design maximizes the diffusion gradient across the sample, typically achieving a 99% buffer exchange in much less time than conventional dialysis methods.

• Hermetically Sealed System: The membrane is permanently bonded to a rigid plastic frame, eliminating the risk of leaks associated with knots or clips used in traditional tubing, and ensuring the absolute security of high-value samples.

• Easy Sample Access: Features multiple gasket-sealed ports that allow for secure sample introduction and total retrieval using a syringe and needle, ensuring zero loss of volume during the process.

Product Specifications

• Catalog Number: 66212

• Sample Volume Range: 3 ml to 12 ml

• MWCO: 2,000 Daltons (2K)

• Membrane Type: Regenerated Cellulose

• Dimensions: Approx. 5 cm x 5 cm

• Purity: Certified free of sulfur and heavy metals.

• Packaging: Available as individual cassettes or in packs of 8.

Key Benefits & Applications

• Peptide Purification: The primary choice for desalting synthetic peptides or small recombinant proteins where higher MWCO membranes would result in significant sample loss.

• Buffer Exchange & Desalting: Provides a controlled environment for the removal of urea, Guanidine-HCl, or salts following chemical modification or protein extraction.

• Equilibrium Studies: Suitable for ligand-binding assays and protein-protein interaction studies where maintaining a precise molecular environment is required.

• Sample Security: The rigid frame design protects the membrane from physical damage during handling, making it ideal for processing expensive or one-of-a-kind biological materials.

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