Thermo Scientific Aspire RP30 Desalting Tips 96 Purifications

Thermo Scientific 990-01 Aspire RP30 Desalting Tips (C18 Reverse-Phase)

The Thermo Scientific 990-01 Aspire RP30 is a high-performance micro-scale purification tip designed for the rapid desalting and concentration of peptides and proteins. Utilizing a proprietary reverse-phase chromatography resin, these tips effectively bind hydrophobic biomolecules while allowing salts, buffers, and other polar contaminants to be washed away. Optimized for "StageTip" style workflows, the Aspire RP30 is critical for preparing samples for Mass Spectrometry (MS), HPLC, and capillary electrophoresis, ensuring clean spectra and protecting sensitive analytical columns from salt-induced damage.

Technical Performance and Design

• High-Binding RP Resin: Features a robust reverse-phase stationary phase (RP30) that provides excellent recovery for a wide range of peptide fragments and small proteins.

• Integrated Micro-Column: The resin is securely embedded at the tip orifice, allowing for the processing of low-microliter sample volumes (typically 1–200 µL) with minimal dead volume.

• Excellent Salt Rejection: Engineered to remove high concentrations of urea, salts, and detergents that interfere with ionization in ESI-MS and MALDI-TOF workflows.

• Step-Wise Purification: Designed for a simple "Bind-Wash-Elute" protocol that can be performed manually with a standard pipette or integrated into automated liquid handling systems.

Product Specifications

• Catalog Number: 990-01

• Model: Aspire RP30

• Resin Type: Reverse-Phase (RP) / C18-equivalent

• Tip Volume: 200 µL

• Format: 96 Tips per Rack / 10 Racks per Case

• Compatibility: Universal fit for standard 200 µL manual pipettes and automated workstations.

Key Benefits & Applications

• Mass Spectrometry Preparation: The industry standard for cleaning up digested protein samples (e.g., Tryptic digests) before injection into LC-MS/MS systems.

• Peptide Concentration: Simultaneously desalts and concentrates samples; eluting in a small volume of organic solvent provides a highly concentrated analyte for injection.

• MALDI-TOF Sample Prep: Ensures sharp, high-intensity peaks by removing alkali metal ions that cause peak broadening and adduct formation.

• Improved Column Life: By removing particulate and salt-heavy matrices at the tip level, these tips extend the operational lifespan of expensive nano-LC columns.

Technical Usage Note

For maximum recovery, it is recommended to "prime" the resin with an organic solvent (like 50% Acetonitrile) and equilibrate with an aqueous acid (like 0.1% TFA) prior to sample binding.

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