Thermo Scientific Bottletop Filter 500 ml Rapid Flow 0.2 um 15 Filters

Thermo Scientific Nalgene 595-4520 500 ml Rapid-Flow™ Bottle-Top Filter (0.2 µm PES)

The Thermo Scientific Nalgene 595-4520 is a high-performance, single-use vacuum filtration unit designed for the rapid sterilization of cell culture media, buffers, and biological fluids. Featuring a 500 ml funnel capacity and a 0.2 µm Polyethersulfone (PES) membrane, this filter is engineered for maximum throughput and high flow rates. The proprietary Rapid-Flow™ support column design minimizes membrane distortion under vacuum, ensuring a consistent and fast filtration process. With a 45 mm neck size, this unit screws securely onto most standard media bottles, providing a functionally closed system for aseptic fluid transfer. Every unit is certified sterile, non-cytotoxic, and non-pyrogenic, making it the industry standard for critical laboratory and bioprocessing applications.

Technical Performance and Design

• High-Flux PES Membrane: The 0.2 µm PES membrane is inherently hydrophilic and features a low-protein-binding chemistry, ensuring maximum recovery of high-value proteins, growth factors, and nutrients.

• Rapid-Flow™ Support System: The unique internal geometry provides uniform support across the entire membrane surface, preventing "blinding" and maintaining high flux even with viscous or particulate-heavy solutions.

• Secure 45 mm Thread: Specifically designed to fit 45 mm neck diameter bottles, providing a leak-proof seal that maintains vacuum integrity and prevents environmental contamination during the filtration process.

• Sterility Assurance: Gamma-sterilized to SAL 10⁻⁶ and individually packaged to ensure that each unit is ready for immediate use in aseptic environments.

Product Specifications

• Catalog Number: 595-4520

• Funnel Capacity: 500 ml

• Membrane Type: Polyethersulfone (PES)

• Pore Size: 0.2 µm (Sterilizing Grade)

• Neck Size: 45 mm

• Membrane Diameter: 75 mm

• Sterility: Sterile (Gamma Irradiated, SAL 10⁻⁶)

• Regulatory: USP Class VI compliant; Non-cytotoxic; Non-pyrogenic.

Key Benefits & Applications

• Cell Culture Media Sterilization: The preferred choice for filtering large volumes of aqueous media where high flow rates and low protein binding are essential for maintaining media performance.

• Buffer and Reagent Prep: Provides a fast and reliable method for sterilizing laboratory buffers, salt solutions, and other aqueous reagents prior to use in sensitive assays.

• Aseptic Transfer: The bottle-top design allows for a direct, sterile transfer into the final storage vessel, reducing the number of fluid handling steps and the risk of contamination.

• Laboratory Efficiency: The Rapid-Flow™ technology significantly reduces filtration time, allowing researchers to process more samples with less downtime compared to standard vacuum filters.

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