Thermo Scientific Culture Flask 500 cm2 Nunclon Delta Case of 32 Flasks

Thermo Scientific Nunc TripleFlask Treated Cell Culture Flask – 500 cm² Growth Area, Filter Cap (MPN: 132865)

The Thermo Scientific Nunc 132865 is a high-density, multi-layer cell culture vessel designed for large-scale mammalian cell expansion, bioproduction, and high-throughput drug screening. Utilizing the innovative TripleFlask™ architecture, this single-width flask houses three parallel, horizontal growth decks integrated into a single clear polystyrene shell. This system yields an expansive 500 cm² total active surface area within the footprint of a traditional standard single-layer automation flask, effectively tripling volumetric cell yields without demanding extra incubator shelf space. Treated with proprietary Nunclon™ Delta gas-plasma technology, all three interior decks are certified hydrophilic and optimized for uniform monolayer formation, cell cloning efficiency, and consistent anchorage-dependent cell line attachment.

Technical Performance and Design

• Nunclon™ Delta Certified Surface: Every manufacturing lot is biologically validated with primary cell lines to guarantee robust, uniform cell spreading. This specialized, hydrophilic surface chemistry accelerates cell attachment and proliferation while eliminating well-to-well and deck-to-deck variations.

• 500 cm² Multi-Layer Efficiency: By integrating three horizontal levels connected to a single common fluid column, this design minimizes space requirements. A working medium volume of 100 mL to 200 mL allows for maximum optimization of media usage per square centimeter of growth area.

• Continuous Sterile Gas Exchange: Equipped with a high-density polyethylene (HDPE) screw cap fitted with a built-in 0.22 µm hydrophobic filter membrane. This configuration facilitates continuous, steady gas equilibration with incubator CO₂ atmospheres while acting as an absolute physical barrier against microbial entry or aerosol leakage.

• Equilibrial Liquid Routing: Designed for straightforward fluid handling. Tilting the flask 75 degrees or standing it upright enables natural gravity to distribute a homogeneous cell suspension evenly among the three compartments before moving it into the final flat incubation position.

Product Specifications

• Catalog Number / MPN: 132865

• Manufacturer / Brand: Thermo Scientific / Nunc™ TripleFlask™

• Total Growth Area: 500 cm²

• Recommended Working Volume: 100 mL to 200 mL

• Surface Treatment: Nunclon™ Delta Certified (Adherent Cell Grade)

• Cap Type / Style: Filter Cap with 0.22 µm Membrane

• Material: Crystal-Grade Transparent Polystyrene (PS)

• Sterility Status: Gamma Irradiated Sterile (SAL 10⁻⁶), Certified Non-Pyrogenic and Non-Cytotoxic

Key Benefits & Applications

• Biopharmaceutical Scale-Up: The standard choice for expanding cellular biomass before routing into benchtop bioreactors or scaling up production for viral vector cultivation (e.g., AAV, Lentivirus).

• Incubator Space Consolidation: Maximizes cell manufacturing output within facilities by condensing three single-layer T-175 flasks into the tight spatial envelope of one TripleFlask module.

• Streamlined Harvesting Protocols: Minimizes labor overhead by allowing researchers to trypsinize, wash, and harvest cells from three separate growth layers simultaneously using standard pipetting techniques.

• Contamination Risk Reduction: By reducing the required manipulations (opening, pouring, and capping) to just one vessel instead of three separate traditional flasks, the risk of accidental environmental exposure is reduced by 66%.

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