Thermo Scientific Culture Flask 500 cm2 Nunclon Vent Cap Case of 32 Flasks

Thermo Scientific Nunc 500 cm² TripleFlask with Vent Cap (132867)

The Thermo Scientific 132867 TripleFlask is a high-efficiency cell culture vessel designed for the large-scale expansion of anchorage-dependent cell lines. By utilizing a unique three-layer parallel growth surface design, the TripleFlask provides a total culture area of 500 cm² within the external dimensions of a conventional T-175 flask. Each internal layer is treated with the Nunclon Delta surface, a proprietary cell-culture treatment that ensures consistent cell attachment and uniform growth across all levels. The unit features a 0.2 µm hydrophobic vent cap to facilitate continuous, sterile gas exchange while maintaining pH stability and protecting against contamination. Delivered sterile and certified non-pyrogenic, the 132867 is the industry standard for researchers performing protein expression, virus production, and high-volume cell harvesting where incubator space is at a premium.

Technical Performance and Design

• Triple-Layer Surface Technology: Integrates three horizontal growth surfaces into a single vessel, effectively tripling the yield per flask and significantly reducing the labor associated with passaging and harvesting large cell populations.

• Nunclon Delta Treatment: Every growth surface is energy-treated to create a consistent, hydrophilic environment that promotes the rapid attachment and proliferation of a wide variety of primary and immortalized cell lines.

• High-Efficiency Gas Exchange: The integrated vent cap features a high-flow 0.2 µm PTFE membrane, allowing for equalized CO2 and O2 concentrations across all three layers while acting as a reliable microbial barrier.

• Precision Liquid Handling: Designed with an internal manifold that ensures even distribution of media and dissociation enzymes across all layers when the flask is positioned correctly, simplifying the workflow for complex scale-up tasks.

Product Specifications

• Catalog Number: 132867

• Total Growth Area: 500 cm²

• Format: Triple-Layer Flask (TripleFlask)

• Surface Treatment: Nunclon Delta (Certified for Cell Culture)

• Cap Style: Vent Cap (0.2 µm Hydrophobic Membrane)

• Material: High-Clarity Polystyrene (PS)

• Sterility: Sterile (Gamma Irradiated to SAL 10^-6)

• Purity: Certified Non-Pyrogenic and Non-Cytotoxic.

Key Benefits & Applications

• Biopharmaceutical Production: Ideal for producing large quantities of recombinant proteins and monoclonal antibodies in adherent culture systems.

• Viral Vector Expansion: Used extensively in gene therapy research for the expansion of HEK293 and other cell lines used in viral packaging.

• Incubator Space Optimization: Allows laboratories to maximize their existing equipment capacity by providing 3x the surface area in a standard incubator footprint.

• Streamlined Harvesting: Reduces the number of individual flasks handled by technicians, decreasing the risk of contamination and lowering operational costs associated with media and labor.

Expiration: 2022-12.

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