Thermo Scientific Culture Flask Triple with Filter Cap 500 cm2 Case of 32 Flasks

Thermo Scientific Nunc TripleFlask Treated Cell Culture Flask – 500 cm² Growth Area, Filter Cap, Barcoded (MPN: 132920)

The Thermo Scientific Nunc 132920 is a high-density, multi-layer cell culture vessel designed for large-scale mammalian cell expansion, automated drug screening, and bioproduction scale-up. Utilizing the specialized TripleFlask™ architecture, this single-width flask houses three parallel, horizontal growth surfaces integrated into a single crystal polystyrene shell. This configuration delivers an expansive 500 cm² total active growth area within the exact physical footprint of a traditional single-layer 175 cm² flask. Treated with proprietary Nunclon™ Delta gas-plasma technology, all three internal decks are certified hydrophilic and optimized for maximum cell cloning efficiency and uniform monolayer formation. This specific automated-grade model features a straight neck configuration and comes pre-printed with a high-contrast Code 128 barcode matrix for integrated inventory tracking.

Technical Performance and Design

• Pre-Printed Code 128 Barcodes: Features a large, scratch-resistant Code 128 barcode label applied directly to the flask body. This enables immediate identification and automated sample logging when running through robotic track lines or barcode-scanning incubator banks.

• Nunclon™ Delta Certified Surface: Every manufacturing lot undergoes strict validation testing across multiple cell lines to ensure robust, uniform cell spreading. This proprietary hydrophilic chemistry accelerates attachment and proliferation while completely eliminating deck-to-deck performance variance.

• Continuous Sterile Gas Exchange: Equipped with a high-density polyethylene (HDPE) screw cap containing a built-in 0.22 µm hydrophobic filter membrane. This facilitates smooth, continuous gas equilibration with incubator CO₂ atmospheres while functioning as an absolute barrier against microbial ingress.

• Automation-Friendly Straight Neck: The straight neck profile is engineered to give automated liquid handling needles, automated aspiration probes, and standard pipettes unhindered entry into the common internal fluid chamber.

Product Specifications

• Catalog Number / MPN: 132920

• Manufacturer / Brand: Thermo Scientific / Nunc™ TripleFlask™

• Total Growth Area: 500 cm² (Condenses three T175 growth layers into one unit)

• Recommended Working Volume: 100 mL to 200 mL

• Surface Treatment: Nunclon™ Delta Certified (Adherent Cell Grade)

• Closure Type: Filter Cap (0.22 µm Membrane)

• Neck Profile: Straight Neck

• Tracking Matrix: Integrated Code 128 Barcode Label

• Sterility Status: Gamma Irradiated Sterile (SAL 10⁻⁶), Certified Non-Pyrogenic and Non-Cytotoxic

Key Benefits & Applications

• High-Throughput Automation Skids: Designed for smooth integration into high-throughput screening (HTS) robotics, automation gripper arms, and automated liquid handlers that manage cellular seed lines.

• Incubator Chamber Consolidation: Maximizes cell manufacturing throughput by condensing the spatial footprint of three individual T175 flasks into a single TripleFlask shell, saving up to 66% of physical shelf space.

• Biopharmaceutical Scale-Up: Highly efficient for generating bulk biomass before downstream bioreactor inoculation or expanding cell lines for large-scale viral vector production (e.g., AAV, Lentivirus).

• Equilibrial Liquid Routing: Designed for clean, fast fluid handling. Tilting the flask to an upright position allows gravity to distribute the cell suspension evenly across all three compartments before moving it flat into the incubator.

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