Thermo Scientific LabTek Chamber Slide with Cover 4 Well Glass Pack of 8 Slides

Thermo Scientific 177399 Lab-Tek Chamber Slide – 4-Well, Glass Slide, Sterile

The Thermo Scientific 177399 Lab-Tek Chamber Slide is a premier solution for cell culture and imaging, designed to allow researchers to grow, fix, stain, and view cells on a single microscope slide. This 4-well system features a removable polystyrene chamber securely attached to a high-quality 25 x 75 mm soda-lime glass slide. The glass surface is specially treated to be non-fluorescent and provides an optimal substrate for the attachment and growth of anchorage-dependent cells. The 177399 includes a fitted cover to maintain sterility and minimize evaporation during incubation. Once the culture period is complete, the chamber can be easily removed using the included removal tool (if provided) or a standard laboratory technique, allowing the slide to be coverslipped for high-resolution microscopy. Certified sterile and non-pyrogenic, these chamber slides are essential for immunofluorescence, in situ hybridization, and specialized cell-based assays.

Technical Performance and Design

• Integrated Growth-to-Analysis Workflow: Eliminates the need for cell harvesting or cytocentrifugation by allowing cells to be processed and imaged directly on the growth surface, maintaining native cell morphology.

• Soda-Lime Glass Substrate: Provides superior optical clarity and low background auto-fluorescence, making it the industry standard for confocal and fluorescence microscopy.

• Removable Multi-Well Chamber: The 4-well configuration allows for the simultaneous testing of multiple variables or controls on a single slide, significantly reducing reagent consumption and processing time.

• Medical-Grade Sealing: The chamber is attached to the slide using a biocompatible silicone gasket/adhesive that ensures a leak-proof seal during incubation while permitting clean removal for mounting.

Product Specifications

• Catalog Number: 177399

• Number of Wells: 4

• Slide Material: Soda-Lime Glass

• Well Capacity: Approximately 0.5 mL – 0.9 mL per well

• Growth Area: 1.8 cm² per well

• Sterility: Sterile (Gamma Irradiated)

Key Benefits & Applications

• Immunocyto-Chemistry (ICC): The 4-well format is perfect for comparing different primary antibodies or concentrations on a single slide under identical conditions.

• Confocal Microscopy: The thin, high-clarity glass slide is optimized for oil immersion lenses and high-magnification imaging of intracellular structures.

• Viral Infection Studies: Ideal for monitoring viral cytopathic effects (CPE) or protein localization in infected cell lines over time.

• Permanent Archiving: Once stained and coverslipped, Lab-Tek slides can be stored for years, making them a vital tool for pathology and longitudinal research studies.

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