Thermo Scientific Microplate 384 Well 120 ul White Case of 30 Plates

Thermo Scientific Nunc 142762 Optical Bottom Microplate – 384-Well, White, Cell Culture Treated

The Thermo Scientific Nunc 142762 is a 384-well optical bottom microplate engineered specifically for high-throughput cell-based luminescence assays and microscopic imaging. Designed with an advanced two-part construction, the plate features opaque white polystyrene walls bonded to an ultra-clear, ultra-thin polymer base. The proprietary Nunclon Delta surface treatment modifies the flat bottom to be highly hydrophilic, promoting robust cellular attachment and growth for adherent mammalian cell lines. Supplied sterile and complete with a lid, this plate allows researchers to culture cells and read luminescent signals in the exact same vessel, streamlining workflows and reducing consumable waste.

Technical Performance and Design

• Optical Clear Bottom: The flat, virgin crystalline polymer base provides uncompromised optical clarity for bottom-reading spectrophotometers and automated phase-contrast or fluorescent imaging systems.

• White Opaque Walls: The white upper structure actively maximizes luminescent signal reflection back to the detector while completely blocking well-to-well optical crosstalk and minimizing background autofluorescence.

• Optimized Well Geometry: Features a rounded-square well shape with a total capacity of 120 µL. This unique geometry eliminates capillary wicking in the corners while providing a larger target area for automated liquid dispensers.

• Nunclon Delta Surface: The certified tissue culture (TC) treatment guarantees consistent, uniform adherent cell monolayers across all 384 wells, ensuring highly reproducible biological data.

Product Specifications

• Catalog Number: 142762

• Format: 384-Well Optical Bottom Plate (with Lid)

• Material: White Polystyrene Frame / Clear Polymer Base

• Volume Capacity: 120 µL Total (10 µL to 100 µL Working Volume)

• Surface Treatment: Nunclon Delta (Cell Culture Treated)

• Sterility: Sterile

Key Benefits & Applications

• Cell-Based Luminescence: The absolute gold standard for running high-throughput ATP cell viability assays, luciferase reporter gene assays, and bioluminescence imaging.

• High-Throughput Screening (HTS): The standard SBS/ANSI footprint and precise pinchbar design ensure seamless handling by robotic grippers, automated plate washers, and liquid handling workstations.

• Reagent Conservation: The 384-well format allows researchers to miniaturize costly cell-based assays down to 10–50 µL working volumes without sacrificing optical pathlength or assay sensitivity.

• Workflow Efficiency: Eliminates the need to transfer cell lysates from a clear culture plate to a solid white reading plate, directly preventing sample loss and volumetric pipetting errors.

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