Thermo Scientific Microplate 6 Well 3 ml Individually Sealed Case of 75 Plates

Thermo Scientific Nunc 6-Well Cell Culture Microplate (140675)

The Thermo Scientific 140675 is a premium 6-well microplate designed for the high-performance cultivation of anchorage-dependent cell lines. Manufactured from high-clarity, medical-grade virgin polystyrene, these plates feature the proprietary Nunclon Delta surface treatment—a permanent, chemically modified surface that ensures optimal cell attachment, uniform spreading, and consistent growth rates. Each well provides a generous 9.6 cm² growth area, specifically designed with a flat-bottom geometry to facilitate high-resolution microscopic observation. Delivered sterile and certified non-pyrogenic, the 140675 is the industry standard for researchers performing primary cell culture, toxicity screening, and molecular biology assays where high-quality cell health is paramount.

Technical Performance and Design

• Nunclon Delta Surface Treatment: Every well is precision-treated to create a hydrophilic surface that promotes rapid cell attachment and exceptional confluence across the entire growth area.

• Raised Well Rims: Features elevated rims around each well that nest into a specialized lid design, effectively minimizing evaporation and reducing the risk of cross-contamination between wells.

• Distortion-Free Optics: The flat-bottom design and high-purity polystyrene construction provide glass-like clarity, allowing for sharp visual inspection and high-quality imaging of cell morphology.

• Automation-Ready ANSI Footprint: Designed to meet standard microplate dimensions, ensuring physical compatibility with robotic liquid handlers, plate readers, and automated incubators.

Product Specifications

• Catalog Number: 140675

• Format: 6-Well Plate

• Growth Area: 9.6 cm² per well

• Working Volume: 3.0 ml per well

• Well Geometry: Round Well / Flat Bottom

• Material: High-Clarity Virgin Polystyrene (PS)

• Sterility: Sterile (Gamma Irradiated to SAL 10^-6)

• Purity: Certified Non-Pyrogenic and Non-Cytotoxic.

Key Benefits & Applications

• Adherent Cell Expansion: Optimized for the growth of sensitive mammalian cell lines, including HEK293, CHO, and primary human fibroblasts.

• Transfection & Transduction: The 6-well format provides the necessary cell density and surface area for efficient viral or chemical-mediated gene transfer experiments.

• Drug Discovery & Toxicity: Ideal for short-term and long-term dose-response studies where clear visual monitoring of cell viability is required.

• Consistent Scalability: Part of the Nunc cell culture family, allowing for predictable results when scaling cultures from 96-well plates up to 6-well formats and T-flasks.

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Expiration: 2026-05.