Thermo Scientific Microplate 96 Well 300 ul PS U-Bottom 90 Plates

Thermo Scientific Nunc 168136 Microplate – 96-Well, U-Bottom, Nunclon Delta Treated

The Thermo Scientific Nunc 168136 is a high-performance 96-well microplate engineered for demanding cell culture and assay applications. Fabricated from optically clear, virgin polystyrene, this plate features a U-shaped (round) bottom in each of its 300 µL wells. This geometry is exceptionally useful for agglutination assays, cell pelleting, and gentle washing steps, as the lack of sharp corners prevents cell damage and allows for complete liquid retrieval. The critical feature of this plate is the Nunclon Delta surface treatment. This fully validated, proprietary plasma-treatment process creates a highly hydrophilic surface that actively promotes the attachment, spreading, and proliferation of adherent mammalian cell lines. Supplied sterile and complete with a low-evaporation lid, the 168136 provides a flawless, ready-to-use platform for high-throughput screening, immunology, and cellular biology workflows.

Technical Performance and Design

• Nunclon Delta Surface Treatment: Transforms standard polystyrene into a highly hydrophilic surface, guaranteeing optimal cell attachment and uniform growth across all 96 wells without the need for biological coatings.

• U-Bottom Geometry: The rounded well floor eliminates dead corners, significantly enhancing the efficiency of cell washing and making it the ideal shape for hemagglutination and colorimetric assays.

• Superior Optical Clarity: The premium polystyrene construction ensures excellent light transmission and low background fluorescence, making it fully compatible with automated plate readers and inverted microscopes.

• Automation Compatibility: Designed with a standardized ANSI/SLAS footprint, raised well rims to prevent cross-contamination, and a high-flange profile that interfaces perfectly with robotic liquid handlers and stackers.

Product Specifications

• Catalog Number: 168136

• Format: 96-Well, U-Bottom (Round)

• Volume: 300 µL Total Capacity (50–250 µL Working Volume)

• Material: Clear Polystyrene (PS)

• Surface Treatment: Nunclon Delta (Cell Culture Treated)

• Sterility & Accessories: Sterile, Lid Included

Key Benefits & Applications

• Adherent Cell Culture: The certified Nunclon Delta surface provides an immediate, out-of-the-box solution for the robust growth of fibroblasts, epithelial cells, and other anchorage-dependent lines.

• Agglutination Assays: The round bottom encourages cells and beads to settle predictably in the center of the well, forming clear, easy-to-read buttons or lattices.

• Sample Recovery: The U-shaped contour prevents the pipettor tip from locking against the well floor, ensuring near-100% retrieval of expensive reagents or precious cell suspensions.

• Contamination Control: Each plate comes pre-sterilized with its own specialized lid designed to minimize evaporation while maintaining vital gas exchange during long-term incubation.

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