Thermo Scientific Microplate 96 Well 330 ul Immulon 2HB Nonsterile PS 40 Plates

Thermo Scientific 3455 Immulon 2HB Microplate – 96-Well, Clear Polystyrene

The Thermo Scientific 3455 is a 96-well clear polystyrene microplate featuring the proprietary Immulon 2HB (High Binding) surface. Designed specifically for quantitative solid-phase enzyme-linked immunosorbent assays (ELISA) and sensitive colorimetric applications, this non-sterile plate is optimized to bind medium-to-large biomolecules, antibodies, and complexes. The flat-bottom well geometry provides superior optical clarity for precise absorbance readings, while the 330 µL maximum well volume accommodates standard reagent additions and automated wash steps without the risk of fluid spillover.

Technical Performance and Design

• Immulon 2HB Surface: The specially irradiated polystyrene surface provides a significantly increased binding capacity for proteins and hydrophilic molecules compared to standard untreated or medium-binding plates.

• Optical Clarity: Manufactured from premium clear polystyrene, the plate ensures uncompromised transmission of visible light, a critical requirement for accurate and reproducible readings in standard colorimetric plate readers.

• Flat Bottom Geometry: The flat-bottom well design maximizes the horizontal surface area available for antibody/antigen capture while maintaining a constant optical path length across all 96 wells.

• Standard SBS Footprint: Conforms strictly to ANSI/SBS microplate dimensional standards, guaranteeing seamless integration with automated plate washers, multi-channel pipetting stations, and robotic liquid handlers.

Product Specifications

• Catalog Number: 3455

• Format: 96-Well Solid Plate

• Material: Clear Polystyrene

• Well Geometry: Round with Flat Bottom

• Volume Capacity: 330 µL Maximum

• Surface Treatment: Immulon 2HB (High Bind)

• Sterility: Non-Sterile

Key Benefits & Applications

• ELISA and Immunoassays: The industry standard for direct antigen coating or capturing monoclonal/polyclonal antibodies during sandwich ELISAs and complex quantitative diagnostic assays.

• High Signal-to-Noise Ratio: The highly consistent surface treatment ensures uniform protein binding across the entire plate, reducing well-to-well coefficient of variation (CV) values and ensuring reliable data.

• Broad Analyte Compatibility: Excellent for anchoring large proteins, immunoglobulins, and complex biomolecules that possess both hydrophobic and ionic interaction regions.

• Reagent Efficiency: The 330 µL capacity safely accommodates typical 50 µL to 200 µL working volumes while leaving sufficient headspace to prevent cross-contamination during rigorous automated washing protocols.

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