Thermo Scientific Microplate 96 Well Sealed 60 Plates

The Thermo Scientific 442404 Microplate, widely known under the Nunc brand, is a specialized 96-well plate considered the industry standard for quantitative immunoassays. These plates feature a flat bottom design and a total well capacity of approximately 0.4 ml (400 µl).

Key Features & Specifications:

MaxiSorp Surface: The defining characteristic of this plate is its MaxiSorp treatment. This is a modified, highly hydrophilic polystyrene surface that has a very high affinity for molecules with mixed hydrophilic/hydrophobic domains. It is specifically optimized to bind antibodies (IgG) and other proteins efficiently, making it the "gold standard" surface for ELISA (Enzyme-Linked Immunosorbent Assay) applications.

Optical Quality: The Clear Polystyrene construction combined with the Flat Bottom (F-bottom) geometry ensures excellent optical clarity. This is essential for accurate and reproducible light transmission readings in microplate readers (spectrophotometers).

Design: The plates are typically designed with raised rims on the wells to reduce the risk of cross-contamination and are compatible with standard automated equipment.

Common Applications: This plate is the go-to choice for sandwich ELISAs and other solid-phase immunoassays where high sensitivity and consistent protein binding are required to detect low concentrations of analytes.