Thermo Scientific Microplate Nunclon Sphera 96 Well 300 ul PS Case of 8 Plates.

Thermo Scientific 174925 Nunc Nunclon Sphera 96-Well Microplate (Ultra-Low Attachment)

The Thermo Scientific 174925 is a specialized 96-well microplate engineered for advanced 3D cell culture applications, including the generation of spheroids, organoids, and embryoid bodies. Featuring the proprietary Nunclon™ Sphera™ surface—a chemically defined, animal-origin-free hydrogel—this plate virtually eliminates cell-to-surface attachment by blocking the adsorption of extracellular matrix (ECM) proteins. The Round (U) Bottom well geometry promotes rapid cell-to-cell aggregation, ensuring the formation of single, consistent, and highly reproducible spheroids in every well. This plate is an essential tool for drug screening, stem cell research, and cancer biology where mimicking the 3D in vivo environment is critical for data accuracy.

Technical Performance and Design

• Superior Ultra-Low Attachment (ULA): The Nunclon Sphera surface provides a biologically inert environment with minimal protein adsorption, forcing cells to aggregate into 3D structures rather than forming 2D monolayers.

• Optimized Spheroid Formation: The Round (U) Bottom well design utilizes gravity to centralize cells at the base of the well, facilitating the formation of a single, uniform spheroid per well and simplifying downstream imaging and analysis.

• Enhanced Optical Clarity: Manufactured from crystal-grade virgin polystyrene, these clear plates provide excellent light transmission for high-content imaging and automated microscopic monitoring of 3D cultures.

• Consistency and Reproducibility: Rigorously tested to ensure well-to-well consistency in spheroid size and growth kinetics, reducing experimental variability in high-throughput screening (HTS) workflows.

Product Specifications

• Catalog Number: 174925

• Format: 96-Well Round (U) Bottom

• Surface Treatment: Nunclon Sphera (Ultra-Low Attachment)

• Total Well Volume: 300 µl

• Working Volume Range: 50 to 250 µl

• Sterility: Sterile (SAL 10⁻⁶); Non-pyrogenic; Biologically inert (USP Class VI).

• Packaging: Individually Wrapped with Lid (1 per pack / 8 per case)

Key Benefits & Applications

• Cancer Research & Drug Discovery: Ideal for creating 3D tumor spheroids that better simulate tumor microenvironments and drug resistance profiles compared to traditional 2D cultures.

• Stem Cell Biology: Supports the consistent formation of embryoid bodies (EB) from pluripotent stem cells (iPSCs/hESCs) with minimal spontaneous differentiation.

• Organoid Generation: Validated for the establishment of complex 3D structures, including brain, liver, and gut organoids, providing a robust platform for developmental biology.

• Automation Ready: Features a standard ANSI/SLAS footprint for seamless integration with automated liquid handlers, plate readers, and high-content screening (HCS) systems.

Expiration Date: 11/2023

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