Thermo Scientific Microplate UpCell 12 Well Multidish 2 ml Case of 6 Plates

Thermo Scientific™ Nunc™ UpCell™ Temperature-Responsive 12-Well Multidish, 2 mL (174900) – Case of 6

The Thermo Scientific™ Nunc™ UpCell™ 174900 is a highly specialized, cutting-edge cell culture plate engineered to enable the non-enzymatic harvesting of adherent cell populations. Utilizing a proprietary temperature-responsive polymer surface matrix, this 12-well multidish shifts from hydrophilic to hydrophobic states based entirely on thermal conditions, allowing intact cells and delicate extracellular matrix structures to detach naturally. Supplied as a sterile package of 6 plates per case, each flat-bottom well features a 2.0 mL working volume capacity, completely eliminating the need for trypsinization or harsh mechanical scraping to maximize cell viability and preserve critical cell surface proteins.

Technical Performance & Design

• Temperature-Responsive Polymer Matrix:** Chemically grafted with a poly(N-isopropylacrylamide) polymer layer. At standard incubator conditions (37C), the surface is hydrophobic, promoting robust cell attachment and proliferation. When shifted below (32C} (room temperature), the surface instantly transitions to hydrophilic, binding water molecules to gently release adherent cells.
• Enzyme-Free Intact Cell Release:** Eliminates the use of trypsin, dispase, or collagenase digests. Because the cell-to-surface bonds are released thermally rather than enzymatically, fragile cell surface receptors, antigen markers, and membrane proteins remain completely undamaged.
• Continuous Cell Sheet Harvesting:** Retains endogenous cell-to-cell junctions and deposit matrices intact during release. This enables the harvesting of continuous, uniform single-layer "cell sheets" utilizing the included specialized supportive membrane membranes for tissue engineering pipelines.
• Contamination-Defensive Rim Architecture:** Molded with a raised well rim layout coupled with single-position ventilation lids. This geometric design allows smooth gas exchange inside the incubator while providing exceptional protection against ambient splashing and airborne cross-contamination.

Product Specifications

• Model Number / Catalog No:** 174900 (Nunc™ UpCell™ Special Culture Series)
• Primary Manufacturer:** Thermo Fisher Scientific / Nunc™
• Well Plate Layout:** Standard 12-well multidish configuration 4x3 matrix
• Well Bottom Geometry:** Flat Bottom with a clear, optically polished viewing field
• Surface Treatment Class:** UpCell™ Temperature-Responsive Polymer Coating
• Dimensional Footprint:**  3.5 cm^2 Culture Area per well | 2.0 mL Maximum Volumetric Working Capacity per well
• Sterility Specification:** Gamma Radiation Sterilized Baseline (Sterility Assurance Level 10^-6
• Purity Verification:** Certified Non-pyrogenic, endotoxin-free, and highly biocompatible for delicate stem cell lines
• Packaging Configuration:** Case of 6 Plates (Individually blister-wrapped for cleanroom entry compliance, includes specialized supportive membranes for sheet manipulation)

Key Benefits & Applications

• Intact Cell Surface Antigen Preservation:** Highly recommended for downstream flow cytometry, cell sorting, or membrane receptor analysis where trypsinization can cleave or degrade critical extracellular surface proteins.
• Advanced Tissue Engineering and Regenerative Medicine:** Ideal for generating multi-layered cellular sheets, constructing artificial skin grafts, modeling blood vessel linings, and building transplantable corneal tissues without synthetic scaffold matrices.
• Delicate Primary and Stem Cell Isolation:** Minimizes chemical shock and physical handling stress on temperamental primary tissue cultures, patient-derived cell lines, or induced pluripotent stem cells (iPSCs).
• Automated Microscope Imaging Compatibility:** The highly uniform, optical-grade crystalline polystyrene wells permit crisp, high-resolution phase-contrast microscopy or high-content imaging sweeps during thermal detachment phases.

Thermo Scientific™, Nunc™, UpCell™, and Nunclon™ are registered commercial trademarks of Thermo Fisher Scientific Inc. Part number 174900 specifies the 12-well multidish format with a temperature-responsive surface. For standard daily suspension cultures or classical trypsin-driven operations, standard non-treated or Nunclon Delta treated multi-well plates should be utilized instead.