{"product_id":"thermo-scientific-microplate-upcell-12-well-multidish-2-ml","title":"Thermo Scientific Microplate UpCell 12 Well Multidish 2 ml Case of 6 Plates","description":"\u003ch3\u003eThermo Scientific™ Nunc™ UpCell™ Temperature-Responsive 12-Well Multidish, 2 mL (174900) – Case of 6\u003c\/h3\u003e\n\u003cp\u003eThe Thermo Scientific™ Nunc™ UpCell™ 174900 is a highly specialized, cutting-edge cell culture plate engineered to enable the non-enzymatic harvesting of adherent cell populations. Utilizing a proprietary temperature-responsive polymer surface matrix, this 12-well multidish shifts from hydrophilic to hydrophobic states based entirely on thermal conditions, allowing intact cells and delicate extracellular matrix structures to detach naturally. Supplied as a sterile package of 6 plates per case, each flat-bottom well features a 2.0 mL working volume capacity, completely eliminating the need for trypsinization or harsh mechanical scraping to maximize cell viability and preserve critical cell surface proteins.\u003c\/p\u003e\n\u003ch3\u003eTechnical Performance \u0026amp; Design\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTemperature-Responsive Polymer Matrix:** Chemically grafted with a poly(N-isopropylacrylamide) polymer layer. At standard incubator conditions (37C), the surface is hydrophobic, promoting robust cell attachment and proliferation. When shifted below (32C} (room temperature), the surface instantly transitions to hydrophilic, binding water molecules to gently release adherent cells.\u003c\/li\u003e\n\u003cli\u003eEnzyme-Free Intact Cell Release:** Eliminates the use of trypsin, dispase, or collagenase digests. Because the cell-to-surface bonds are released thermally rather than enzymatically, fragile cell surface receptors, antigen markers, and membrane proteins remain completely undamaged.\u003c\/li\u003e\n\u003cli\u003eContinuous Cell Sheet Harvesting:** Retains endogenous cell-to-cell junctions and deposit matrices intact during release. This enables the harvesting of continuous, uniform single-layer \"cell sheets\" utilizing the included specialized supportive membrane membranes for tissue engineering pipelines.\u003c\/li\u003e\n\u003cli\u003eContamination-Defensive Rim Architecture:** Molded with a raised well rim layout coupled with single-position ventilation lids. This geometric design allows smooth gas exchange inside the incubator while providing exceptional protection against ambient splashing and airborne cross-contamination.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eProduct Specifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eModel Number \/ Catalog No:** 174900 (Nunc™ UpCell™ Special Culture Series)\u003c\/li\u003e\n\u003cli\u003ePrimary Manufacturer:** Thermo Fisher Scientific \/ Nunc™\u003c\/li\u003e\n\u003cli\u003eWell Plate Layout:** Standard 12-well multidish configuration 4x3 matrix\u003c\/li\u003e\n\u003cli\u003eWell Bottom Geometry:** Flat Bottom with a clear, optically polished viewing field\u003c\/li\u003e\n\u003cli\u003eSurface Treatment Class:** UpCell™ Temperature-Responsive Polymer Coating\u003c\/li\u003e\n\u003cli\u003eDimensional Footprint:**  3.5 cm^2 Culture Area per well | 2.0 mL Maximum Volumetric Working Capacity per well\u003c\/li\u003e\n\u003cli\u003eSterility Specification:** Gamma Radiation Sterilized Baseline (Sterility Assurance Level 10^-6\u003c\/li\u003e\n\u003cli\u003ePurity Verification:** Certified Non-pyrogenic, endotoxin-free, and highly biocompatible for delicate stem cell lines\u003c\/li\u003e\n\u003cli\u003ePackaging Configuration:** Case of 6 Plates (Individually blister-wrapped for cleanroom entry compliance, includes specialized supportive membranes for sheet manipulation)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Benefits \u0026amp; Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eIntact Cell Surface Antigen Preservation:** Highly recommended for downstream flow cytometry, cell sorting, or membrane receptor analysis where trypsinization can cleave or degrade critical extracellular surface proteins.\u003c\/li\u003e\n\u003cli\u003eAdvanced Tissue Engineering and Regenerative Medicine:** Ideal for generating multi-layered cellular sheets, constructing artificial skin grafts, modeling blood vessel linings, and building transplantable corneal tissues without synthetic scaffold matrices.\u003c\/li\u003e\n\u003cli\u003eDelicate Primary and Stem Cell Isolation:** Minimizes chemical shock and physical handling stress on temperamental primary tissue cultures, patient-derived cell lines, or induced pluripotent stem cells (iPSCs).\u003c\/li\u003e\n\u003cli\u003eAutomated Microscope Imaging Compatibility:** The highly uniform, optical-grade crystalline polystyrene wells permit crisp, high-resolution phase-contrast microscopy or high-content imaging sweeps during thermal detachment phases.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003cp\u003e\u003csmall\u003eThermo Scientific™, Nunc™, UpCell™, and Nunclon™ are registered commercial trademarks of Thermo Fisher Scientific Inc. Part number 174900 specifies the 12-well multidish format with a temperature-responsive surface. For standard daily suspension cultures or classical trypsin-driven operations, standard non-treated or Nunclon Delta treated multi-well plates should be utilized instead.\u003c\/small\u003e\u003c\/p\u003e","brand":"Thermo Scientific","offers":[{"title":"Default Title","offer_id":44498234147068,"sku":"4508","price":285.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0627\/8463\/5132\/files\/thermo-scientific-microplate-upcell-12-well-multidish-2-ml-case-of-6-plates-petri-dishesculture-plates-thermo-scientific-880125.jpg?v=1781969453","url":"https:\/\/lifesciencestrading.com\/products\/thermo-scientific-microplate-upcell-12-well-multidish-2-ml","provider":"Life Sciences Trading","version":"1.0","type":"link"}