Thermo Scientific Nunc Chamber Slide 16 Well with Cover 16 Chamber Slides

Thermo Scientific 178599 Lab-Tek Chamber Slide – 16-Well, Glass Slide, Sterile

The Thermo Scientific 178599 (Nunc Lab-Tek) is a specialized cell culture system designed for the simultaneous cultivation and microscopic analysis of multiple cell populations. This system features a 16-well polystyrene chamber structure securely attached to a premium 25 x 75 mm non-fluorescent soda-lime glass slide. The 16-well configuration is ideal for small-volume high-throughput screening, dose-response studies, and multi-marker immunofluorescence. Each well provides a discrete growth area, and the unique gasketless design ensures a leak-proof seal without the use of toxic adhesives. After cultivation, the chamber can be easily removed using the included separator tool, leaving the cells attached to the glass slide for permanent mounting and high-resolution imaging. Certified sterile and non-pyrogenic, these chamber slides are an essential tool for advanced cell biology and diagnostic pathology.

Technical Performance and Design

• Microscope Glass Growth Surface: The soda-lime glass substrate provides optimal optical clarity and ultra-low auto-fluorescence, which is critical for high-resolution confocal and fluorescence microscopy.

• Removable Chamber Structure: The 16-well chamber can be detached from the slide after cell growth, allowing for the application of a coverslip and long-term archival storage of the specimen.

• Alphanumeric Well Identification: Each well is clearly labeled on the chamber frame, facilitating easy sample tracking and error-free mapping during complex multi-reagent experiments.

• Broad Chemical Compatibility: The glass surface is resistant to common histological fixatives such as formaldehyde, glutaraldehyde, and alcohols, as well as various fluorescent dyes and mounting media.

Product Specifications

• Catalog Number: 178599 (Nunc / Lab-Tek)

• Well Configuration: 16 Wells

• Slide Material: Soda-Lime Glass

• Chamber Material: Polystyrene

• Growth Area: Approx. 0.4 cm² per well

• Suggested Working Volume: 0.1 – 0.2 mL per well

• Sterility: Sterile (SAL 10⁻³)

Key Benefits & Applications

• Immunocytochemistry (ICC): Perfect for staining multiple antibodies on the same slide, ensuring identical processing conditions for various cell markers.

• Dose-Response Assays: The 16-well format allows for a wide range of drug concentrations or treatment time points to be evaluated in a single microscopic field of view.

• Primary Cell Research: Ideal for precious primary cell samples that require small-volume culturing to achieve the necessary cell density for downstream analysis.

• High-Resolution Imaging: Unlike plastic-bottom plates, the glass substrate allows for the use of oil-immersion objectives and short-working-distance lenses without optical distortion.

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